Allele mining in wild barley: finding new exotic genes which control flowering time in the barley nested association mapping (NAM) population HEB-25.

野生大麦等位基因挖掘：在大麦嵌套关联作图 (NAM) 群体 HEB-25 中寻找控制开花时间的新外来基因。

• 批准号: 197422382
• 负责人: Dr. Jochen Kumlehn
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/197422382?language=en
• 关键词: Allele; mining; wild; barley; finding

During the second phase of the PP-9 project, we plan to study the function of major genes controlling flowering time, FTi, in the exotic barley population HEB-25 within the following three work packages, WP.WP1, QTL cloning - We will isolate an exotic gene that controls late flowering in HEB-25 at a newly identified locus on chromosome 4H in collaboration with the group of Prof. Dr. Andy Flavell, University of Dundee. Based on exome capture sequencing data of the 1,420 HEB lines, two top candidate genes, which differentiate between early and late flowering pools of recombinant HEB lines, will be selected and subjected to three transgenic approaches. These involve over-expression, knock-out-knock-down and promoter-reporter assays. Whereas the first two approaches will assist to identify the causal gene that causes the late flowering phenotype, the latter approach will be used to characterize the spatial and temporal expression of the candidate genes.WP2, Allele mining - We will study the variation of allelic effects of the 25 exotic HEB donors at four dominant major FTi genes, Ppd-H1, denso, Vrn-H2 and Vrn-H3. For this, 12 exotic haplotypes of the FTi genes will be cloned from HEB lines and used to generate over-expression transformants in the genomic background of the recessive spring barley Golden Promise genotype. The effects of the transformations on FTi will be studied to identify new allelic variants displaying altered FTi phenotypes.WP3, tress tolerance - We will study the flowering effects of FTi genes on yield and their crosstalk with stress tolerance on a global scale. For this, we will select 48 HEB lines, which reveal variation in flowering time, and, which segregate at four major FTi genes, Ppd-H1, denso, VrnH1 and Vrn-H3 on chromosomes 2H, 3H, 5H and 7H, respectively. Those HEB lines will be tested during two years (2015 and 2016) in field plots under two Treatments - stress versus control - and in four replications at five locations world-wide. Based on the subsequent modelling, we will quantify the impact of the studied FTi genes on yield and stress tolerance. Since the tested environments differ along a latitudal gradient, we may also select favorable exotic FTi alleles in response to day length variation.The external partners, their affiliations and the stresses studied are:1. Prof. Dr. Jason Eglinton, University of Adelaide, Australia, drought stress2. Prof. Dr. Mark Tester, King Abdullah University of Science and Technology, Saudi Arabia, salt stress3. Dr. Michael Baum, ICARDA, Jordan, heat stress4. Dr. William Thomas, James Hutton Institute, Dundee, nitrogen deficiency.

在 PP-9 项目的第二阶段，我们计划在以下三个工作包（WP.WP1、QTL 克隆）中研究外来大麦群体 HEB-25 中控制开花时间的主要基因 FTi 的功能 - 我们将与英国大学 Andy Flavell 教授团队合作，在 4H 染色体上新发现的基因座上分离控制 HEB-25 晚花的外来基因。 邓迪。根据 1,420 个 HEB 品系的外显子组捕获测序数据，将选择区分重组 HEB 品系的早花和晚花库的两个顶级候选基因，并进行三种转基因方法。这些涉及过度表达、敲除-敲低和启动子-报告基因检测。前两种方法将有助于识别导致晚花表型的因果基因，后一种方法将用于表征候选基因的空间和时间表达。WP2，等位基因挖掘 - 我们将研究 25 个外来 HEB 供体在四个显性主要 FTi 基因（Ppd-H1、denso、Vrn-H2 和 Vrn-H3）上的等位基因效应的变化。为此，将从 HEB 品系中克隆 FTi 基因的 12 个外来单倍型，并用于在隐性春大麦 Golden Promise 基因型的基因组背景中产生过度表达转化体。将研究转化对 FTi 的影响，以确定显示改变的 FTi 表型的新等位基因变体。WP3，植株耐受性 - 我们将在全球范围内研究 FTi 基因对产量的开花影响及其与胁迫耐受性的串扰。为此，我们将选择 48 个 HEB 品系，这些品系揭示了开花时间的变化，并且在 2H、3H、5H 和 7H 染色体上的四个主要 FTi 基因 Ppd-H1、denso、VrnH1 和 Vrn-H3 上分别分离。这些 HEB 系将在两年内（2015 年和 2016 年）在两种处理（胁迫与控制）下的田间试验中进行测试，并在全球五个地点进行四次重复测试。基于随后的建模，我们将量化所研究的 FTi 基因对产量和抗逆性的影响。由于测试的环境沿纬度梯度不同，我们还可以选择有利的外来FTi等位基因来响应日长变化。外部伙伴、他们的从属关系和研究的压力是： 1． Jason Eglinton 博士教授，澳大利亚阿德莱德大学，干旱胁迫2。沙特阿拉伯阿卜杜拉国王科技大学 Mark Tester 教授、博士，盐胁迫3。 Michael Baum 博士，ICARDA，约旦，热应激4。威廉·托马斯博士，詹姆斯·赫顿研究所，邓迪，氮缺乏。