RUI: Rational design of cysteinyl peptide ligands as chelators of mercury (II)

RUI：作为汞螯合剂的半胱氨酰肽配体的合理设计（II）

• 批准号: 1011859
• 负责人: Maria Ngu-Schwemlein
• 金额: $ 23.3万
• 依托单位: Winston-Salem State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-15 至 2015-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1011859&HistoricalAwards=false
• 关键词: RUI; Rational; design; cysteinyl; peptide

This award in the Chemical Synthesis (SYN) program in the Division of Chemistry supports work by Professors Maria Ngu-Schwemlein, John Merle, and Sayo Fakayode at Winston-Salem State University to carry out fundamental studies on the rational design and synthesis of peptide derived binding agents for the toxic mercury(II) ion. Evaluating the mercury(II) binding affinity of some cysteine-containing peptides will reveal the selectivity and thermodynamic factors that are important in assessing their mode(s) of interaction. Correlations between the chemical structures of some cysteinyl peptide ligands and their binding activity for mercury(II) will be explored by microcalorimetric measurements, and identification of the binding specificity will be conducted by a combination of theoretical calculations and solid state studies. Additionally, the molecular interactions of these compounds with some biological molecules will be monitored by spectroscopic measurements. Selective and effective mercury(II) binding agents are extremely useful for the development of mercury(II) trapping agents for chelation therapy of mercury poisoning.This interdisciplinary research study will enhance the research environment of the chemistry department at Winston-Salem State University in preparing undergraduates, primarily underrepresented groups at this historically black institution, for entry into advanced degree programs and careers in science. This research may also afford a basis for subsequent research in the environmental and health sciences such as the development of microsensors for monitoring toxic and harmful metal ions in environmental and biological samples. Additionally, this research may provide useful peptides for future application in genetically engineered plants that are useful for phytoremediation of mercury(II) pollution in soil or watersheds.

化学系化学合成（SYN）项目的这一奖项支持温斯顿-塞勒姆州立大学的Maria Ngu-Schwemlein、John Merle和Sayo Fakayode教授的工作，他们开展了关于有毒汞（II）离子的肽衍生结合剂的合理设计和合成的基础研究。 评估汞（II）结合亲和力的一些含半胱氨酸的肽将揭示的选择性和热力学因素，是重要的，在评估其模式（S）的相互作用。 一些半胱氨酰肽配体的化学结构和它们的汞（II）的结合活性之间的相关性将探讨微量热测量，和识别的结合特异性将进行理论计算和固态研究的结合。 此外，这些化合物与一些生物分子的分子相互作用将通过光谱测量来监测。 选择性和有效的汞（II）结合剂是非常有用的汞（II）捕集剂的螯合治疗汞中毒的发展，这一跨学科的研究研究将提高化学系在温斯顿-塞勒姆州立大学的研究环境，在准备本科生，主要是在这个历史上的黑人机构，进入高级学位课程和职业生涯在科学。 该研究也为环境和健康科学的后续研究提供了基础，例如开发用于监测环境和生物样品中有毒有害金属离子的微传感器。 此外，这项研究可能会提供有用的肽，为未来的应用在基因工程植物，是有用的汞（II）污染的土壤或流域的植物修复。