EAGER: Crosslinked Biodegradable Nanoparticles by Thiol-Ene Miniemulsion Reaction

EAGER：通过硫醇-烯细乳液反应交联可生物降解纳米颗粒

• 批准号: 1019227
• 负责人: Chong Cheng
• 金额: $ 5万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2011-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1019227&HistoricalAwards=false
• 关键词: EAGER; Crosslinked; Biodegradable; Nanoparticles; Thiol

1019227Cheng Intellectual Merit Integrating biodegradability with nanostructures has generated nanomaterials with minimized long-term toxicity and important biomedical applications. However, it remains challenging to obtain well-defined biodegradable nanomaterials through simple and robust protocols. This research is focused on a methodology for the synthesis of environmentally benign polymeric nanomaterials via thiol-ene miniemulsion-crosslinking reactions. The PI previously found high reactivity of an allyl-functionalized lactide (LA) monomer in thiol-ene reactions. A well-defined PLA-based copolymer with pendent allyl groups was further obtained by organo-catalyzed ring-opening copolymerization of the functional monomer with LA; transparent miniemulsions were prepared with relatively low surfactant concentrations. A critical question is whether thiol-containing miniemulsion systems have sufficient stability to allow well-controlled templating synthesis. In this exploratory investigation, the PI plans to synthesize PLA-based nanoparticles using the thiol-ene reaction between the PLA-based copolymer and multifunctional thiols within miniemulsion nanodroplets. This is a high-risk approach because it has not been previously explored. However, if successful, results could lead to a new avenue for making biodegradable nanaomaterials. Experimentally, monitored by DLS, stability of the miniemulsion reaction systems will be investigated, and miniemulsion conditions will be optimized to exert precise control over the resulting nanoparticles. DLS, FT-IR, AFM, and TEM will be utilized to characterize the nanoparticles. Broader Impacts The research could lead to extensive synthetic studies of biodegradable nanoparticles with different structural features, as well as detailed investigations of degradation and delivery behaviors of the nanoparticles. Since these biodegradable nanostructures may be used to deliver anti-cancer drugs through nanoencapsulation or conjugation, eventually this project may have societal health-care benefits. A video on nanoparticle synthesis by thiol-ene miniemulsion crosslinking will be prepared as open source material available on the internet (including YouTube) to promote polymer education. Broad dissemination will also be through the 'Passport STEM' program for middle and high school students from Buffalo and surrounding school districts (both public and private schools).

将生物可降解性与纳米结构相结合产生了长期毒性最小的纳米材料和重要的生物医学应用。然而，通过简单和可靠的方案获得定义明确的可生物降解纳米材料仍然具有挑战性。本研究致力于通过硫醇-烯微乳液交联反应合成环境友好型聚合物纳米材料的方法学。PI先前发现烯丙基功能化丙交酯(LA)单体在硫醇-烯反应中具有很高的反应性。通过有机催化功能单体与LA的开环共聚，进一步得到了含烯丙基的聚乳酸基共聚物，制得了表面活性剂浓度较低的透明微乳液。一个关键的问题是，含硫醇的微乳液体系是否具有足够的稳定性，以实现良好控制的模板合成。在这项探索性的研究中，PI计划利用聚乳酸共聚物与微乳液纳米液滴中的多功能硫醇之间的硫醇-烯反应来合成聚乳酸纳米颗粒。这是一种高风险的方法，因为它以前从未被探索过。然而，如果成功，结果可能会为制造可生物降解的纳米材料开辟一条新的途径。在实验上，通过DLS监测，将研究微乳液反应体系的稳定性，并将优化微乳液条件，以对生成的纳米颗粒进行精确控制。利用DLS、FT-IR、AFM、TEM等手段对纳米颗粒进行了表征。更广泛的影响这项研究可能导致对具有不同结构特征的可生物降解纳米颗粒的广泛合成研究，以及对纳米颗粒的降解和输送行为的详细研究。由于这些可生物降解的纳米结构可能被用来通过纳米胶囊或结合来输送抗癌药物，最终该项目可能会产生社会医疗保健效益。将在互联网(包括YouTube)上制作一段关于通过硫醇-烯细乳液交联合成纳米颗粒的视频，作为可供使用的开源材料，以促进聚合物教育。还将通过针对布法罗和周边学区(包括公立和私立学校)的初中生和高中生的‘Passport STEM’计划进行广泛传播。