Collaborative Research: Molecular Determinants of Power Inputs and Outputs of Synchronous Flight Muscle in Vivo

合作研究：体内同步飞行肌功率输入和输出的分子决定因素

• 批准号: 1022471
• 负责人: Thomas Daniel
• 金额: $ 28.03万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1022471&HistoricalAwards=false
• 关键词: Collaborative; Research; Molecular; Determinants; Power

Project Title: Collaborative Research: Molecular Determinants of Power Inputs and Outputs of Synchronous Flight Muscle In Vivo Principal Investigators: Irving, Thomas C, and Thomas L. Daniel NSF Project Numbers: IOS 1022058 and 1022471 All moving animals, from humans to flying insects, operate with muscles that not only cyclically generate force, they do so while generating significant heat. This research project is aimed at understanding the molecular basis and physiological consequences of temperature dependent force generation in muscle. As with many other biological rate processes, the speed and power output of muscle is strongly influenced by temperature. Surprisingly, heat generation by the muscles that power flight in Hawkmoths show a large temperature gradient, with more superficial muscles operating at cooler temperatures than deeper, more insulated, muscles. This temperature gradient has profound functional consequences and is likely a general result for many moving creatures. The researchers will examine the notion that thermal gradients lead to functional gradients. Thus, deeper warmer, muscle subunits may serve as power generators driving locomotion whereas cooler subunits may act as elastic energy storage systems. All of this function operates with protein motors that will be examined from the molecular level to the fully intact muscle in a moving animal. A mix of high-speed x-ray imaging methods and whole muscle force and energy measurement methods will be used to tackle this problem. The combination of heat generation by the volume of muscle in humans and other animals, combined with processes that dissipate heat suggests that temperature gradients may be more common than historically assumed. Thus it is likely a general phenomenon that the temperature dependent function of muscle will vary spatially within a single muscle group. In addition, the flight muscle of Manduca sexta will provide a new model system for understanding muscle function in animals in general. A result of this project will be development and refinement of x-ray diffraction methods to probing muscle function in vivo.

项目名称：合作研究：体内同步飞行肌肉动力输入和输出的分子决定因素主要研究者：Irving， Thomas C，和Thomas L. Daniel NSF项目编号：IOS 1022058和1022471所有运动的动物，从人类到飞行昆虫，都是通过肌肉进行操作的，这些肌肉不仅会周期性地产生力量，而且还会产生大量的热量。本研究项目旨在了解肌肉中温度依赖性力产生的分子基础和生理后果。与许多其他生物速率过程一样，肌肉的速度和能量输出受到温度的强烈影响。令人惊讶的是，为飞蛾飞行提供动力的肌肉产生的热量显示出很大的温度梯度，与更深、更绝缘的肌肉相比，更浅层的肌肉在更低的温度下工作。这种温度梯度具有深远的功能影响，可能是许多移动生物的普遍结果。研究人员将研究热梯度导致功能梯度的概念。因此，深层温暖的肌肉亚单位可以作为驱动运动的发电机，而较冷的亚单位可以作为弹性能量储存系统。所有这些功能都是通过蛋白质马达来运作的，我们将从分子水平到运动动物的完整肌肉来研究蛋白质马达。高速x射线成像方法和全肌肉力量和能量测量方法的混合将用于解决这个问题。人类和其他动物的肌肉体积产生的热量与散热过程的结合表明，温度梯度可能比历史上认为的更为普遍。因此，在单个肌肉群中，肌肉的温度依赖功能可能会发生空间变化，这可能是一种普遍现象。此外，该研究还将为进一步了解动物的肌肉功能提供一个新的模型系统。这个项目的结果将是发展和改进x射线衍射方法来探测体内肌肉功能。