Soybean Root Hairs: A Model for Single-Cell Plant Biology

PI: Gary Stacey（密苏里大学）合作PI：程建林（密苏里大学）和徐东（密苏里大学）合作者：David Koppenaal（太平洋西北国家实验室）和Ljiljana Pa？a- toliæ（太平洋西北国家实验室）系统生物学是对生物系统中所有组成部分相互作用的方式进行全面、定量分析的学科。最终的目标是一个新的，预测细胞功能的观点，取代旧的描述性的理解。然而，即使有了先进的技术，由于各种各样的问题，系统生物学还没有实现它的承诺，其中最重要的是生物系统的整体复杂性。该项目通过研究一种植物细胞类型，即根毛，对有益的固氮细菌日本慢根瘤菌感染的反应，降低了这种复杂性。目前的项目建立在过去的工作基础上，该工作已将大豆根毛细胞作为基因组研究的理想模型。目标是特别关注控制感染过程的调控网络，从而成功建立固氮共生关系。除了与系统生物学的相关性之外，这项工作有望对植物细胞功能、根系发育和固氮过程产生新的见解，这在农艺学和生态学上具有重要意义。根毛是植物固氮过程中对细菌反应的第一个部位。因此，在系统水平上识别植物的响应途径可以为调控作物固氮提供新的途径。该项目将产生序列数据，这些数据将通过GenBank和我们的项目网站（www.soyroothair.org）以及大豆知识库（www.SoyKB.org）公开。后者被设计为一个综合资源，以容纳，可视化和分析所有类型的大豆功能基因组数据。该项目具有实质性的推广和教育组成部分，与大学合作解决K-12，本科生，研究生和博士后培训问题。

PI: Gary Stacey (University of Missouri)Co-PIs: Jianlin Cheng (University of Missouri) and Dong Xu (University of Missouri)Collaborators: David Koppenaal (Pacific Northwest National Laboratory) and Ljiljana Pa?a-Toliæ (Pacific Northwest National Laboratory)Systems biology is the comprehensive, quantitative analysis of the manner in which all components of a biological system interact. The ultimate goal is a new, predictive view of cellular function, supplanting the older descriptive understanding. However, even with the availability of advanced technologies, systems biology has yet to achieve its promise due to a variety of issues, not the least of which is the overall complexity of biological systems. The project reduces this complexity by studying the response of a single, plant cell type, the root hair, to infection by the beneficial nitrogen fixing bacterium, Bradyrhizobium japonicum. The current project builds on past work that has developed the soybean root hair cell as an ideal model for genomic study. The goal is to focus specifically on the regulatory networks that control the infection process leading to the successful establishment of a nitrogen fixing symbiosis. Beyond its relevance to systems biology, the work promises to yield new insights into plant cellular function, root development and the nitrogen fixation process, which is of great agronomic and ecological importance. The root hair is the first site of plant response to bacteria during the nitrogen fixation process. Therefore, identifying the plant response pathways at a systems level could provide new avenues for manipulating nitrogen fixation in crop plants. The project will yield sequence data that will be made publicly available through GenBank and through our project website (www.soyroothair.org), as well as the Soybean Knowledgebase (www.SoyKB.org). The latter is being designed as a comprehensive resource to house, visualize and analyze all types of soybean functional genomic data. The project has a substantial outreach and educational component that partners with the University to address K-12, undergraduate, graduate and postdoctoral training.