SGER: Repeat and BAC-End Sequencing of Soybean

一张高分辨率的大豆种群物理地图。Williams 82基因组目前正在构建中。然而，这一努力受到大豆基因组的大小、复杂性和有限的知识结构的阻碍。这个探索性研究项目的小额资助将解决bac末端序列和公共重复序列数据库的迫切需要，以帮助地图组装和注释。待开发的序列资源将为更好地估计大豆基因组的基因和重复序列含量提供依据。bac端序列将被整合到物理图谱中，并用于锚定基因和遗传标记。该项目的具体目标是：1]通过对随机生成的25000个质粒克隆进行单次测序，生成一个重复序列数据库；2]对20000个bac末端进行测序；3]对序列进行注释；4]将数据纳入Fingerprint Contig （FPC）数据库，供社区访问（http://www.genome.arizona.edu/）。序列数据将每周发布到GenBank的GSS部门（http://www.ncbi.nlm.nih.gov/dbGSS/）。一个强大的大豆基因组图谱将促进大豆功能基因组学工具的广泛发展。一个额外的结果将是招募年轻科学家，他们将大豆视为一个令人兴奋的研究系统，可以在此基础上建立自己的职业生涯。这种临界质量的增加将带来许多切实的好处，包括保持大豆作为一种农业作物的生存能力，并通过确保安全和可用的粮食供应来促进国土安全。

A high-resolution, physical map of the soybean cv. Williams 82 genome is currently under construction. However, this effort is hampered by the size, complexity, and limited knowledge of the structure of the soybean genome. This Small Grant for Exploratory Research project will address the urgent need for BAC-end sequences and a public repeat sequence database to aid in map assembly and annotation. The sequence resources to be developed will provide a better estimate of the gene and repeat content of the soybean genome. The BAC-end sequences will be integrated into the physical map, as well as used to anchor genes and genetic markers.The specific goals of the project are to 1] generate a repeat sequence database by single-pass sequencing of 25,000 randomly generated plasmid clones, 2] sequence 20,000 BAC-ends, 3] annotate the sequence, and 4] incorporate the data into the Fingerprint Contig (FPC) database for community access (http://www.genome.arizona.edu/). Sequence data will be released to the GSS division of GenBank (http://www.ncbi.nlm.nih.gov/dbGSS/) on a weekly basis.A robust map of the soybean genome will facilitate the development of a wide range of functional genomics tools for soybean. An additional outcome will be the recruitment of young scientists who will see soybean as an exciting research system on which they can build their careers. This increase in critical mass will have many tangible benefits, including preserving the viability of soybean as an agriculture crop and contributing to homeland security by insuring a safe and available food supply.