Posttranscriptional Regulation, a Novel Mechanism for Ion Channel Regulation and Dendritic Excitability

转录后调节，离子通道调节和树突兴奋性的新机制

• 批准号: 1026527
• 负责人: Kimberly Raab-Graham
• 金额: $ 45万
• 依托单位: University of Texas at Austin
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-08-15 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1026527&HistoricalAwards=false
• 关键词: Posttranscriptional; Regulation; Novel; Mechanism; Ion

The protein mTOR (or mammalian Target Of Rapamycin) regulates cell growth, proliferation, motility, energy status, and survival. In the brain, mTOR activity is important for learning and memory. Dr. Raab-Graham has shown that mTOR suppresses the local translation of the voltage-gated potassium channel Kv1.1. Many voltage-gated ion channels are important for proper neuronal communication. How their expression is regulated during learning and memory is unknown. With a strong background in ion channels, cell biology and biochemistry her lab will identify the physiological and molecular mechanism for mTOR suppression of Kv1.1. She hypothesizes that several different types of ion channels are under the local control of mTOR. Discovering how mTOR suppress Kv1.1 translation may lead to identifying a network of functionally related genes that are inhibited by mTOR.This project provides training for undergraduates, graduates, and postdoctoral students in the multidisciplinary project addressing the molecular, biochemical, and physiological mechanisms of learning and memory, an intrinsic benefit to society. Members of the PI's laboratory participate in the public outreach program, Memory Matters. This event educates the community by engaging them in scientific demonstrations of our research, in a manner designed to educate and inform non-scientists. Through this involvement, future generations of scientists are learning to serve as liaisons between the scientific community and the general public. In addition, this project will help support the development of new technology to detect changes in electrical signaling in neuronal dendrites. This endeavor is part of an international collaboration with scientists in Japan. Furthermore, the academic training ground provided by this project will concentrate on retention of women in science, by encouraging all students to be rigorous scientists, to serve as scientific liaisons, and to learn how to effectively balance the demands of a scientific career with other life obligations.

蛋白质mTOR（或哺乳动物雷帕霉素靶）调节细胞生长、增殖、运动、能量状态和存活。在大脑中，mTOR活性对学习和记忆很重要。Raab-Graham博士已经证明mTOR抑制电压门控钾通道Kv1.1的局部翻译。许多电压门控离子通道对于正常的神经元通信是重要的。它们的表达在学习和记忆过程中是如何调节的尚不清楚。凭借在离子通道，细胞生物学和生物化学方面的强大背景，她的实验室将确定Kv1.1的mTOR抑制的生理和分子机制。她假设几种不同类型的离子通道在mTOR的局部控制下。发现mTOR如何抑制Kv1.1翻译可能会导致识别mTOR抑制的功能相关基因网络。本项目为本科生，研究生和博士后学生提供多学科项目的培训，解决学习和记忆的分子，生物化学和生理机制，对社会有内在的好处。PI实验室的成员参加了公共外展计划，记忆问题。该活动通过让他们参与我们研究的科学演示来教育社区，旨在教育和告知非科学家。通过这种参与，未来几代科学家正在学习充当科学界和公众之间的联络人。此外，该项目将有助于支持新技术的开发，以检测神经元树突中电信号的变化。这项奋进是与日本科学家进行国际合作的一部分。此外，该项目提供的学术培训场所将侧重于留住科学界的妇女，鼓励所有学生成为严谨的科学家，充当科学联络员，并学习如何有效地平衡科学事业的要求与其他生活义务。