Molecular dynamics of RNA model systems studied by time-resolved optical and IR spectroscopy (A07)

RNA的结构和构象动力学对其生物学功能的重要作用日益被认识到。因此，我们开发了系统和方法，以获得各种调控RNA的功能动态的时间和地点分辨图像。特定的光谱标记允许监测折叠和杂交的动态。基于偶氮苯或螺吡喃单元或光笼环状RNA的可光切换的RNA配体系统提供了必要的空间和时间分辨率来触发和观察这些过程并阐明其潜在的机制。

The vital role of RNA structure and conformational dynamics for its biological functionality is increasingly recognized. Therefore we develop systems and methodologies to obtain a time- and site-resolved picture of the functional dynamics of various regulatory RNAs. Specific spectroscopic labels allow to monitor folding and hybridization dynamics. Photoswitchable RNA-ligand-systems based on azobenzene or spiropyran units or photocaged circularized RNAs provide the necessary spatial and temporal resolution to trigger and observe these processes and to elucidate the underlying mechanisms.