Elucidation of mechanism of molecular dynamics recognition by RNA machineries involved in the thermostable RNA maturation

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Structural and functional analyses of tRNA splicing enzyme (EndA) from ultra-small archaeon, ARMAN-2, revealed that ARMAN-2 specific loop is involved in the broad substrate specificity of ARMAN-2 EndA. On the other hands, structural, biochemical and genetic analyses of tRNA (m2G10/m22G10) methyltransferase (arcTrm11) in the hyperthermophilic archaeon Thermococcus kodakarensis showed that the crystal structure of arcTrm11 consists of two domains, N-terminal THUMP domain which is involved in the RNA recognition and C-terminal Rossman fold-type catalytic domain, and that arcTrm11 gene is essential for cell viability at high temperature.