Connecting Trace Elements and Metalloenzymes Across Marine Biogeochemical Gradients

连接海洋生物地球化学梯度中的微量元素和金属酶

• 批准号: 1031271
• 负责人: Carl Lamborg
• 金额: $ 83.79万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1031271&HistoricalAwards=false
• 关键词: Connecting; Trace; Elements; Metalloenzymes; Across

Two scientists from Woods Hole Oceanographic Institution will determine the role of enzymatic activity in the cycling of trace metals. Specifically the researchers would address the following questions: (1) degradation of sinking particulate organic material in the Tropical North Pacific can be influenced by the ability of microbes to synthesize zinc proteases, which in turn is controlled by the abundance or availability of zinc, and (2) methylation of mercury is controlled, in part, by the activity of cobalt-containing enzymes, and therefore the supply of labile cobalt to the corrinoid-containing enzymes or co-factors responsible for methylation. To attain their goal, they will collect dissolved and particulate samples for trace metals and metalloenzymes from three stations along a biogeochemical gradient in the Tropical North Pacific (along 150°W from 18°N to the equator). Sinking particles from metal clean sediment traps will also be obtained. The samples will also be used to carry out shipboard incubation experiments using amendments of metals, metal-chelators, B12, and proteases to examine the sensitivity and metal limitation of heterotrophic, enzymatic degradation of organic matter within the oceanic "Twilight Zone" (100-500 m). This study will result in a novel metaproteomic/metalloenzyme datasets that should provide insights into the biogeochemical cycling of metals, as well as co-limitation of primary productivity and controls on the export of carbon from the photic zone. In terms of broader impacts, an online metaproteomic data server would be created so the community can have access to the raw data files generated by this research. It is anticipated that the current public school outreach activities in Cleveland, Ohio in which the proponents are involved will be continued. During this effort, the proponents plan to use grow-out incubation experiments to teach students about the role of nutrients in phytoplankton growth. One graduate student would be supported and trained by this project.

来自伍兹霍尔海洋研究所的两名科学家将确定酶活性在微量金属循环中的作用。 具体而言，研究人员将解决以下问题：（1）在热带北太平洋，下沉颗粒有机物质的降解可能受到微生物合成锌蛋白酶的能力的影响，而锌蛋白酶又受到锌的丰度或可用性的控制，（2）汞的甲基化部分受到含钴酶活性的控制，并因此将不稳定的钴供应给负责甲基化的含类咕啉的酶或辅因子。 为了实现这一目标，他们将从热带北太平洋地球化学梯度（从18°N到赤道沿150°W）沿着三个站点收集溶解和微粒样品，以寻找痕量金属和金属酶。 还将获得来自金属清洁沉积物捕集器的沉降颗粒。这些样品还将用于进行船上培养实验，使用金属、金属螯合剂、B12和蛋白酶的改良剂，以检查海洋“阴阳极区”（100-500米）内有机物的异养酶降解的敏感性和金属限制。 这项研究将产生一种新的元蛋白质组学/金属酶数据集，该数据集应提供对金属的地球化学循环的见解，以及初级生产力的共同限制和对透光区碳出口的控制。 就更广泛的影响而言，将创建一个在线元蛋白质组数据服务器，以便社区可以访问这项研究产生的原始数据文件。 预计目前在俄亥俄州克利夫兰市的公立学校外展活动将继续进行，其中包括支持者。 在这项工作中，支持者计划使用生长孵化实验来教学生营养物质在浮游植物生长中的作用。 一名研究生将得到该项目的支持和培训。

项目成果

Abundant nitrite-oxidizing metalloenzymes in the mesopelagic zone of the tropical Pacific Ocean

• DOI: 10.1038/s41561-020-0565-6
• 发表时间: 2020-05
• 期刊: Nature Geoscience
• 影响因子: 18.3
• 作者: M. Saito;M. McIlvin;D. Moran;A. Santoro;C. Dupont;P. Rafter;Jaclyn K. Saunders;D. Kaul;C. Lamborg;Marian B. Westley;F. Valois;J. Waterbury

Hydrothermal trace metal release and microbial metabolism in the northeastern Lau Basin of the South Pacific Ocean

• DOI: 10.5194/bg-18-5397-2021
• 发表时间: 2021-10-06
• 期刊: BIOGEOSCIENCES
• 影响因子: 4.9
• 作者: Cohen, Natalie R.;Noble, Abigail E.;Saito, Mak A.
• 通讯作者: Saito, Mak A.

Minimal cobalt metabolism in the marine cyanobacterium Prochlorococcus

• DOI: 10.1073/pnas.2001393117
• 发表时间: 2020-07-07
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Hawco, Nicholas J.;McIlvin, Matthew M.;Saito, Mak A.
• 通讯作者: Saito, Mak A.