Droplet Based Quantitative Analysis of Single-Cell Cytosol in Large Cell Populations

基于液滴的单细胞溶质定量分析在本项目中将创建一种新的单细胞分析装置，用于检测人类红细胞内的化合物。目前单细胞分析的最新技术是流式细胞术。虽然流式细胞术可以快速分析许多细胞的胞浆，但它不能探测到很多种化合物。在这项研究中，将使用塑料微流控设备来裂解细胞，将细胞内容物封装在带有荧光染料的液滴中，然后检测细胞内存在多少目标分子。塑料微流控装置将被制造出来，然后用于检测人体红细胞中的离子、酶和小化合物的水平。该项目将支持不同学科的一名研究生和一名本科生研究人员，以促进协作学习。这项工作将允许学生在展示成果的同时，在专业会议和研讨会上与他人互动。研究人员还将在当地一所K-12学校开设短期课程，并将为学生提供半天的研究人员实验室之旅，以帮助提高学生对科学技术的兴趣。

Droplet based quantitative analysis of single-cell cytosol in large cell populations In this project will be created a new single-cell analysis device for detecting compounds inside human red blood cells. The current state of the art for single cell analysis is flow cytometry. Although flow cytometry may quickly analyze the cytosol of many cells, it cannot probe for very many kinds of compounds. In this research, a plastic microfluidic device will be used to lyse the cells, encapsulate the contents of the cell in a droplet with a fluorescent dye and then detect how much of the target molecule was present inside the cell. The plastic microfluidic device will be fabricated and then used for the detection of ion, enzyme, and small compound levels in human red blood cells. The project will support a graduate student and an undergraduate researcher of differing disciplines to promote collaborative learning. The work will allow the students to interact with others at professional conferences and workshops while presenting results. The researchers will also give a short class at a local K-12 school, and will offer a half day tour of the researchers' labs to the students to help promote interest in science and technology.