EAGER: A Hybrid Nano-Bioprinting System for Tissue Engineering

EAGER：用于组织工程的混合纳米生物打印系统

• 批准号: 1038769
• 负责人: Alisa Clyne
• 金额: $ 11.94万
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1038769&HistoricalAwards=false
• 关键词: EAGER; Hybrid; Nano; Bioprinting; System

The research objective of this EArly-Concept Grant for Exploratory Research (EAGER) is to understand how nanobioprinting affects cell viability and function. The effect of two parameters will be explored. 1. Nanoparticle size and composition; and 2. Nanoparticle location relative to the cell. Through these objectives, the fundamental understanding of cell-nanoparticle interactions in the bioprinting process will be advanced. This will lead towards advanced studies of nanobioprinting and closer to realizing large scale production of nano-functionalized tissue engineering structures for transplantation and drug discovery. The intellectual merit of this research lies in discovery of nanoparticle interaction with micron-scale cells in nanomanufacturing processes, and how this interaction translates into biological outcomes. By understanding fundamental mechanisms, nanoparticles can be manufactured and used in ways which minimize cellular effects. Most importantly, these studies will enable scale-up of micro- and macro-scale robust, viable tissue engineering constructs with integrated nanostructures. The dynamic manipulation and tracking of cells and bioactive factors within these structures has potential to transform tissue engineering.The broader impact of the research project is in its potential to enhance knowledge of nanoparticle biochemical and biomechanical effects, in particular how nanoparticles and cell interact in the bioprinting system. This has a wide reach in nanomanufacturing for medicine, since nanoparticles interact with mechanosensitive tissues (vasculature, bones, lungs) when used for imaging or cancer treatment. Discovery of nanoparticle parameters that minimize negative interaction between nanoparticles and cell mechanics could create safer, more effective nanotherapy. The broader impacts of the project's educational program are to show mechanical engineers how nanotechnology can be applied to improve healthcare, as well as to inspire future engineers through cutting edge technology in a social context.

这个探索性研究早期概念基金（EAGER）的研究目标是了解纳米生物打印如何影响细胞活力和功能。本文将探讨两个参数的影响。1. 纳米颗粒的大小和组成；和2。纳米颗粒相对于细胞的位置。通过这些目标，对生物打印过程中细胞-纳米颗粒相互作用的基本理解将得到推进。这将导致纳米生物打印的先进研究，并更接近于实现用于移植和药物发现的纳米功能化组织工程结构的大规模生产。本研究的智力价值在于发现纳米粒子在纳米制造过程中与微米级细胞的相互作用，以及这种相互作用如何转化为生物学结果。通过了解基本机制，纳米颗粒可以以最小化细胞效应的方式制造和使用。最重要的是，这些研究将使集成纳米结构的组织工程结构在微观和宏观尺度上的大规模扩展成为可能。这些结构中的细胞和生物活性因子的动态操作和跟踪具有改变组织工程的潜力。该研究项目更广泛的影响在于它有可能增强纳米颗粒生物化学和生物力学效应的知识，特别是纳米颗粒和细胞如何在生物打印系统中相互作用。这在医药纳米制造中具有广泛的影响，因为纳米颗粒在用于成像或癌症治疗时与机械敏感组织（脉管系统、骨骼、肺）相互作用。纳米粒子参数的发现可以最大限度地减少纳米粒子与细胞力学之间的负相互作用，从而创造出更安全、更有效的纳米疗法。该项目的教育计划的更广泛影响是向机械工程师展示纳米技术如何应用于改善医疗保健，以及通过社会背景下的尖端技术激励未来的工程师。