Interactive signal transfer in the leaf apoplast between host and pathogen during successful infection

成功感染期间宿主和病原体之间叶质外体中的交互信号传递

• 批准号: 19985606
• 负责人: Professor Dr. Hubert Heinrich Felle
• 金额: --
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19985606?language=en
• 关键词: Interactive; signal; transfer; leaf; apoplast

The aim of this project is to unravel electrophysiological changes in compatible interactions of barley with pathogenic and mutualistic fungi. In leaves of different barley genotypes, which are susceptible to infection by biotrophic (e.g. Blumeria graminis) or hemibiotrophic fungi (e.g. Bipolaris sorokiniana], as well as in leaves of super-susceptible transgenic barley (expressing the constitutively activated RACBG15V), and in leaves from Piriformospora indica-infested plants, a novel non-invasive micro-probe technique is applied to measure in vivo the interactive signal transfer between host and pathogen directly within the apoplast. Ion-selective microelectrodes are inserted into substomatal cavities to detect changes in apoplastic ion activities that occur through the activity of the pathogen, the host or both within minutes, hours or days after infection without disturbing either of the partners. Through nano-infusion of selected effectors the action of both host and pathogen will be influenced and monitored continuously. Special emphasis is put on the question how the pathogen overcomes defence measures of the host and/or how these measures are avoided, and how transporters of organic substrates are manipulated or reprogrammed before haustoria are formed.

本项目的目的是揭示大麦与病原真菌和互惠真菌相容性相互作用的电生理变化。在不同大麦基因型的叶片中，其易受活体营养型（例如，小麦白粉病菌）或半活体营养型真菌（例如，小离蠕孢），以及在超易感转基因大麦（表达组成型激活的RACBG 15 V）的叶中，和在来自印度梨形孢菌侵染的植物的叶中，一种新的非侵入性微探针技术被应用于直接在质外体内测量宿主和病原体之间的相互作用信号传递。将离子选择性微电极插入气孔下腔，以检测在感染后几分钟、几小时或几天内通过病原体、宿主或两者的活动发生的质外体离子活动的变化，而不干扰任何一方。通过纳米输注选定的效应物，宿主和病原体的作用将受到影响和持续监测。特别强调的问题是病原体如何克服防御措施的主机和/或如何避免这些措施，以及如何运输有机基质的操纵或重新编程吸器形成之前。