EAGER: Nanoparticle Control of Microbial Development on Ceramic Surfaces

EAGER：陶瓷表面微生物发育的纳米颗粒控制

• 批准号: 1043137
• 负责人: Ian Nettleship
• 金额: $ 19.77万
• 依托单位: University of Pittsburgh
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-15 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1043137&HistoricalAwards=false
• 关键词: EAGER; Nanoparticle; Control; Microbial; Development

NON-TECHNICAL DESCRIPTION: Bacteria prefer to exist as surface biofilms in which the bacteria form organized colonies surrounded by a protective extracellular matrix that makes them very resistant to disinfection. The tenacity of biofilms can affect medical outcomes by infecting the surfaces of implants and medical devices that must be subsequently removed. They also have a profound effect on public health by transmitting waterborne and respiratory diseases through water distribution systems. For example, a recent study has shown that pathogenic strains of mycobacteria, commonly transmitted by contaminated water, are highly enriched in household showerheads and may be linked to a rise in pulmonary infections in the developed world. This study is examining the effect of small amounts of antibacterial nanoparticles on the attachment of mycobacteria to ceramic surfaces prior to the development of mature biofilms and consequent resistance to disinfection. The fundamental information obtained can be used to develop strategies for the design and manufacture of biofilm resistant, ceramic water purification membranes to be used in water treatment plants in the developed world and ceramic point-of-use filters designed for households in the less developed world.TECHNICAL DETAILS: A unique combination of ceramics processing science and microbiology is being used to study the effect of antibacterial silver nanoparticles on the attachment of mycobacteria to ceramic surfaces. A controlled deposition method is used to vary the concentration and spatial distribution of the silver nanoparticles on the ceramic surfaces. Then the surfaces are exposed to flowing water containing different strains of mycobacteria and the effect of the silver nanoparticles on the attachment of the bacteria is observed and quantified using confocal optical microscopy, electron microscopy and image analysis. The information is then used to develop biofilm resistant ceramic surfaces. Additionally, the project participants are mentoring undergraduate students participating in service learning experiences that provide product development and engineering services to non-governmental organizations that make low-cost ceramic water filters in poor communities in the less developed world.

非技术描述：细菌更喜欢作为表面生物膜存在，其中细菌形成被保护性细胞外基质包围的有组织的菌落，这使得它们对消毒非常耐受。 生物膜的韧性可以通过感染随后必须去除的植入物和医疗设备的表面来影响医疗结果。 它们还通过配水系统传播水传播疾病和呼吸道疾病，对公共卫生产生深远影响。 例如，最近的一项研究表明，通常通过污染水传播的分枝杆菌致病菌株在家庭中高度富集，可能与发达国家肺部感染的增加有关。 这项研究正在研究少量抗菌纳米颗粒对分枝杆菌在成熟生物膜形成之前附着在陶瓷表面上的影响，以及由此产生的耐消毒性。 所获得的基本信息可用于制定设计和制造抗生物膜陶瓷水净化膜的策略，这些膜将用于发达国家的水处理厂，以及为欠发达国家的家庭设计的陶瓷使用点过滤器。技术参数：陶瓷加工科学和微生物学的独特组合正在被用来研究抗菌银纳米颗粒对附着在分枝杆菌到陶瓷表面。 采用可控沉积方法改变陶瓷表面上银纳米颗粒的浓度和空间分布。 然后将表面暴露于含有不同分枝杆菌菌株的流水中，并使用共聚焦光学显微镜、电子显微镜和图像分析来观察和定量银纳米颗粒对细菌附着的影响。 然后，该信息用于开发抗生物膜的陶瓷表面。 此外，项目参与者正在指导参与服务学习经验的本科生，为在欠发达国家贫困社区生产低成本陶瓷水过滤器的非政府组织提供产品开发和工程服务。