RNA Dynamics by Solution and Solid State Deuterium NMR

通过溶液和固态氘 NMR 进行 RNA 动力学分析

• 批准号: 1050702
• 负责人: Gabriele Varani
• 金额: $ 69.93万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1050702&HistoricalAwards=false
• 关键词: RNA; Dynamics; Solution; Solid; State

The objective of this project is to study dynamics in RNA by applying innovative magnetic resonance methods of solid and solution state NMR. The central hypothesis underlying this project is that motion is an essential aspect of RNA function: therefore, understanding the biological function of nucleic acids requires knowledge of the degree to which motions modulate molecular recognition by proteins and small molecules. By understanding its motions, the project will further understanding of the molecular recognition principles. The new tools to study functional dynamics in nucleic acids developed through this project will be broadly applicable to other nucleic acid systems. Because the project is highly interdisciplinary, spanning biophysics, chemistry, molecular biology and pharmacology, it provides many opportunities for scientific collaborations and for exposing undergraduate and graduate students to interdisciplinary research, and for the training of under-represented minority students.

该项目的目的是通过应用固体和溶液状态NMR的创新磁共振方法来研究RNA的动力学。该项目的核心假设是运动是RNA功能的一个重要方面：因此，理解核酸的生物学功能需要了解运动调节蛋白质和小分子识别的程度。通过了解它的运动，该项目将进一步了解分子识别原理。 通过该项目开发的研究核酸功能动力学的新工具将广泛适用于其他核酸系统。由于该项目是高度跨学科的，跨越生物物理学、化学、分子生物学和药理学，它提供了许多机会进行科学合作，让本科生和研究生接触跨学科研究，并培训代表性不足的少数民族学生。