Functional Analyses of SR1, A Calcium/Calmodulin-Regulated Transcription Factor, and VOZs, Vascular Plant One Zinc Finger Proteins, In Plant Immunity

知识专长：植物病原体（细菌、真菌和病毒）等胁迫物造成粮食作物的巨大损失。了解植物对这种压力的反应机制并产生免疫力对于减少这种损失至关重要。钙是所有真核生物中的关键信号分子，几十年来一直与植物免疫有关，许多研究揭示了细胞钙水平响应病原体攻击的快速和暂时变化。然而，钙如何调节参与植物免疫的途径知之甚少。该项目涉及一个由钙调节的转录因子小家族;这些因子首先在植物中发现，然后在动物中发现。其中一个因子称为SR 1，是植物免疫的负调节因子。SR 1与另外两个转录因子相互作用。本项目的目标是利用遗传学、细胞生物学、生物化学、分子生物学和生物信息学等工具来研究SR 1相互作用蛋白在植物免疫中的生物学作用。利用SR 1相互作用转录因子的单突变体和双突变体分析植物的防御反应，研究SR 1中各保守结构域的作用和功能意义，实现SR 1及其相互作用转录因子靶标的全面鉴定。总体而言，该项目将有助于理解植物免疫中钙调节转录因子的功能。更广泛的影响：从该项目获得的知识将与农业重要植物相关，因为钙调节转录因子在作物中高度保守;更好地了解SR 1及其相互作用蛋白的功能，将在减少粮食作物腐败方面具有潜在的应用价值，感染植物病原体。该项目将通过在遗传学、生物信息学、细胞和分子生物学的各个方面培训高中生、本科生、一名研究生和一名博士后研究员，并鼓励他们参加国家科学会议，将研究与教学结合起来。

Intellectual merit:Stressors such as plant pathogens (bacteria, fungi and viruses) cause enormous losses of food crops. Understanding the mechanisms by which plants respond to such stressors and develop immunity is critical to reducing such losses. Calcium, a key signaling molecule in all eukaryotes, has been implicated in plant immunity for decades, and numerous studies have revealed rapid and temporary changes in cellular calcium levels in response to pathogen attacks. However, how calcium regulates pathways involved in plant immunity is poorly understood. This project concerns a small family of transcription factors that are regulated by calcium; these factors were first discovered in plants and then in animals. One of these factors, named SR1, is a negative regulator of plant immunity. SR1 interacts with two other transcription factors. The goal of this project is to use genetic, cell biological, biochemical, molecular, and bioinformatics tools to study the biological roles of SR1-interacting proteins in plant immunity. Single and double mutants of SR1-interacting transcription factors will be used to analyze plant defense responses, the roles and functional significance of various conserved domains in SR1 will be studied, and global identification of targets of SR1 and its interacting transcription factors will be implemented. Overall, this project will contribute to understanding the functions of a calcium-regulated transcription factor in plant immunity.Broader Impacts: The knowledge obtained from this project will be relevant to agriculturally important plants as the calcium-modulated transcription factors are highly conserved in crop plants; a better understanding of the function of SR1 and its interacting proteins will have potential applications in reducing the food crop spoilage that results from infection with plant pathogens. This project will integrate research and teaching by training high school students, undergraduate students, a graduate student and a postdoctoral researcher in various aspects of genetics, bioinformatics, cell and molecular biology and by encouraging their participation in national scientific meetings.