Collaborative proposal: Regulation of pre-mRNA splicing by light signals

合作提案：光信号调控前mRNA剪接

• 批准号: 2014542
• 负责人: Anireddy Reddy
• 金额: $ 28万
• 依托单位: Colorado State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2014542&HistoricalAwards=false
• 关键词: Collaborative; proposal; Regulation; pre; mRNA

The perception of light is one of the most fundamental ways that plants interact with the environment to alter growth and maximize capture of sunlight. This project will address how environmental light signals perceived by the phytochrome photoreceptors regulate gene expression to affect plant growth and productivity. This project will train the next generation of scientists preferentially from underrepresented groups, who will gain considerable experience in developing new tools in agricultural biotechnology to improve food security, human health, and environmental sustainability. Development and dissemination of educational toolkits will greatly expand educational opportunities to high school students in scientific research. Presentations in biology classes of local K-12 schools will not only teach young students about genetically modified organisms (GMOs), but also improve communication and teaching skills of participating researchers. This will expose a diverse group of K-12 students to a modern research facility, introduce them to the scientific method in a fun and engaging way and inspire them to choose a scientific career path.This project will elucidate poorly understood mechanisms by which phytochromes regulate gene expression at both transcriptional and post-transcriptional levels. Alternative splicing (AS) is a highly regulated process that enhances the diversity of mRNAs encoded in the genome. Previous work by this group showed that this process is also affected by light signals mediated through the photoreceptor phytochrome B (phyB), thereby providing a novel mechanism by which plants can rapidly respond to light signals. The goals of the project are to: 1) Understand the biochemical mechanisms of regulation of splicing by the SFPS-RRC1-SWAP1 splicing module, and 2) Investigate how phyB regulates splicing through the SFPS-RRC1-SWAP1 module under light. The project will use biochemical, molecular, genetic and genomic approaches to elucidate the early events by which phytochromes directly affects alternative splicing in response to light.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

对光的感知是植物与环境相互作用以改变生长并最大限度地捕获阳光的最基本方式之一。本项目将研究光敏色素光感受器感知的环境光信号如何调节基因表达，从而影响植物生长和生产力。该项目将培训下一代科学家，优先来自代表性不足的群体，他们将在开发农业生物技术新工具以改善粮食安全、人类健康和环境可持续性方面获得大量经验。教育工具包的开发和传播将大大扩大高中学生在科学研究方面的教育机会。当地K-12学校生物课上的演讲不仅将向年轻学生讲授转基因生物，还将提高参与研究人员的沟通和教学技能。这将使一群不同的K-12学生接触到现代化的研究设施，以有趣和引人入胜的方式向他们介绍科学方法，并激励他们选择科学的职业道路。该项目将阐明光敏色素在转录和转录后水平调节基因表达的机制。选择性剪接（AS）是一个高度调控的过程，增强了基因组中编码的mRNA的多样性。该小组先前的工作表明，这一过程也受到光受体光敏色素B（phyB）介导的光信号的影响，从而提供了一种新的机制，植物可以快速响应光信号。该项目的目标是：1）了解SFPS-RRC 1-SWAP 1剪接模块调节剪接的生化机制，2）研究phyB如何在光照下通过SFPS-RRC 1-SWAP 1模块调节剪接。该项目将使用生物化学，分子，遗传学和基因组学的方法来阐明光敏色素直接影响选择性剪接的早期事件，以应对light.This奖项反映了NSF的法定使命，并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

A high-resolution single-molecule sequencing-based Arabidopsis transcriptome using novel methods of Iso-seq analysis.

• DOI: 10.1186/s13059-022-02711-0
• 发表时间: 2022-07-07
• 期刊: Genome biology
• 影响因子: 12.3

Decoding co-/post-transcriptional complexities of plant transcriptomes and epitranscriptome using next-generation sequencing technologies

使用下一代测序技术解码植物转录组和表观转录组的共/转录后复杂性

• DOI: 10.1042/bst20190492
• 发表时间: 2020
• 期刊: Biochemical Society Transactions
• 影响因子: 3.9
• 作者: Anireddy S.N. Reddy;Jie Huang;Naeem H. Syed;Asa Ben-Hur;Suomeng Dong;Lianfeng Gu
• 通讯作者: Lianfeng Gu

SWAP1-SFPS-RRC1 splicing factor complex modulates pre-mRNA splicing to promote photomorphogenesis in Arabidopsis.

• DOI: 10.1073/pnas.2214565119
• 发表时间: 2022-11
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Kathare, Praveen Kumar;Xin, Ruijiao;Ganesan, Abirama Sundari;June, Viviana M.;Reddy, Anireddy S. N.;Huq, Enamul
• 通讯作者: Huq, Enamul