Synthesis and Applications of Fluorescent GPI Conjugates

荧光 GPI 偶联物的合成及应用

• 批准号: 1053848
• 负责人: Zhongwu Guo
• 金额: $ 27万
• 依托单位: Wayne State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1053848&HistoricalAwards=false
• 关键词: Synthesis; Applications; Fluorescent; GPI; Conjugates

The Chemical Synthesis Program of the Chemistry Division supports Professor Zhongwu Guo of Wayne State University to prepare GPI conjugates of small molecule dyes by chemical synthesis using the para-methoxybenzyl group for permanent hydroxyl protection as well as GPI-fluorescent protein conjugates by enzyme-catalyzed ligation of synthetic GPIs and proteins. The resulting GPI conjugates will be then utilized to study GPI anchorage of proteins to the cell membrane.This work would improve our skills and tool box for obtaining complex and important biomolecules and advance our fundamental knowledge about how cell surface proteins are attached to the cell membrane, a process that plays a pivotal role in many biological and pathological processes. The proposed work will have a significant impact on the fields of chemistry and biological sciences, including the development of new therapeutic strategies targeting at proteins on the cell surface. This project also helps educate undergraduate and graduate students and train highly demanding experts in the fields of synthetic and carbohydrate chemistry and glycobiology.

化学系的化学合成项目支持韦恩州立大学的郭忠武教授通过化学合成使用对甲氧基苄基进行永久羟基保护来制备小分子染料的GPI缀合物，以及通过合成GPI和蛋白质的酶催化连接来制备GPI-荧光蛋白缀合物。 GPI偶联物将用于研究GPI在细胞膜上的锚定作用，这一工作将提高我们获得复杂和重要生物分子的技能和工具箱，并推进我们对细胞表面蛋白质如何附着在细胞膜上的基础知识，这一过程在许多生物和病理过程中起着关键作用。拟议的工作将对化学和生物科学领域产生重大影响，包括开发针对细胞表面蛋白质的新治疗策略。该项目还有助于教育本科生和研究生，并培养合成和碳水化合物化学和糖生物学领域的高要求专家。