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Fluorescent enhancement of the nitrogen vacancy center in nanoscale diamond for bioimaging applications

用于生物成像应用的纳米金刚石中氮空位中心的荧光增强

基本信息

批准号：
10205094
负责人：
Abraham Wolcott
金额：
$ 10.84万
依托单位：
SAN JOSE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-10 至 2023-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10205094
关键词：
Acids Address Aging Alcohols Amines Applied Research Area Atomic Force Microscopy Biological Biosensing Techniques Cancer Detection Cancer cell line Carbon Cells Cessation of life Chemical Models Chemicals Chemistry Collaborations Confocal Microscopy Deposition Developing Countries Diagnostic Diamond Electron Microscopy Elements Engineering Environment Feedback Fluorescence Fluorescence Microscopy Gases Geometry Goals Growth High temperature of physical object Image Image Enhancement Investigation Knowledge Laboratories Laser Scanning Confocal Microscopy Lasers Lead Location Malignant Neoplasms Measurement Metals Methodology Microscopy Modality Modeling Modification Molecular Molecular Conformation Morphology Movement Nanosphere Nanostructures Nitrogen Noise Oxides Pattern Phase Photons Population Positioning Attribute Process Production Property Protocols documentation Radial Radiation Reaction Research Research Personnel Resolution Roentgen Rays Route Sampling Scheme Science Scientist Self Efficacy Signal Transduction Silicon Silicon Dioxide Siloxanes Spectroscopy, Fourier Transform Infrared Spectrum Analysis Stains Structure Sulfhydryl Compounds Surface Suspensions Synchrotrons Techniques Temperature Sense Thermometry Thick Time Tissues Universities Work absorption base bioimaging cancer imaging cancer statistics cancer type cellular imaging covalent bond density design detection method electric field fluorescence imaging fluorophore fundamental research imaging study light scattering magnetic field metallicity model building multimodality nanodiamond nanomaterials nanoparticle nanoscale pancreatic cancer cells particle plasmonics pressure protocol development self assembly simulation synchrotron radiation tool tool development undergraduate student

项目摘要

Project Summary/Abstract In this body of work the Wolcott laboratory will develop chemical methodologies for the functionalization of nanoscale diamond with the aim of increasing the fluorescent rate of the nitrogen vacancy center (NVC) via plasmonics. We are focusing our expertise to understand chemical reactivity at the high-pressure high- temperature diamond surface and investigate the mechanisms for diamond-metal nanostructure self-assembly. Advancements in bioimaging with fluorescent nanodiamonds (FNDs) are hindered by a lack of robust chemistry to modify their surface. Further, no advances for increasing the NVC fluorescent rate have been realized. In total, these challenges have resulted in poor colloidal stability, poor targeting in staining protocols and no simple routes to enhance FND emission rates. We will address these challenges to advance the surface chemistry of nanoscale diamond, build coherent models of chemical reactivity and increase the efficacy for self-assembly with metallic nanostructures. The nitrogen vacancy center in 25-100 nm crystallites have shown fluorescent enhancement, but only with tedious movement of these structures by atomic force microscopy which is incompatible with cell imaging. A major goal of the study is to understand how tuning the surface chemistry will drive self-assembly, how dielectric layer thickness effects fluorescent rates and the production of FND-metallic constructs with robust properties for bioimaging. Further, we envision using these FND constructs in fluorescent imaging studies with pancreatic cancer cell lines. Our aims include both fundamental studies and applied fluorescence imaging investigations. The goals of the proposed work include: 1) modification of the FND surface with wet chemical and gas phase chemistry, (2) application of the modified FND for metallic nanostructure assembly and (3) the fundamental mechanisms of NVC fluorescence enhancement for imaging applications. Our surface chemistry will focus on covalent bond formation of low-Z elements such as nitrogen and silicon oxide to the ND surface. To probe the surface we will use overlapping techniques that are laboratory and synchrotron-based spectroscopies. Techniques include dynamic light scattering, FTIR, wavelength dependent X-ray photoelectron spectroscopy (XPS) and near edge X-ray absorption fine structure (NEXAFS). Surface information will then be used to direct our chemical methodology and protocol development. The covalent moieties will then act as the molecular anchors to drive their assembly with metal nanostructures and to grow metallic shells. FND emission properties will be investigated with confocal microscopy to determine fluorescent rates and radiative lifetimes. Finite-difference time-domain simulations will guide our chemistry and establishes a target of a 150x fold increase for the NVC fluorescent rate. This work will impact fundamental and applied research where the NVC is used for magnetic and electric field imaging, thermometry and long-term bioimaging applications. PI-Wolcott is an expert in diamond surface chemistry and the use of nanoparticles for biological staining. This project will be supported by the active collaborations with nanomaterial expert Prof. Nicholas Melosh at Stanford University and surface scientist Dr. Dennis Nordlund at the Stanford Synchrotron Radiation Lightsource (SSRL). The prime location of San Jose State University (SJSU) in the Bay Area provides unique opportunities for science to be accomplished at near-by research centers such as SSRL and The Molecular Foundry at Lawrence Berkeley National Laboratory. A highly motivated team of undergraduate researchers in the Wolcott laboratory at SJSU are taking full advantage of this environment and are advancing the initial aims of this proposal as detailed in the Research Strategy.

项目总结/文摘