Novel Biophotonic Probes to Monitor Cellular Metabolism of Nucleosides

监测核苷细胞代谢的新型生物光子探针

• 批准号: 1066107
• 负责人: Thomas Gray
• 金额: $ 59.2万
• 依托单位: Case Western Reserve University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-07-15 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1066107&HistoricalAwards=false
• 关键词: Novel; Biophotonic; Probes; Monitor; Cellular

1066107GrayNucleosides and their corresponding triphosphates are essential building blocks needed for proper cellular function. An underrepresented area of study is the development and application of emissive biosensors to monitor nucleoside transport and metabolism in living cells. Toward this goal, nucleosides will be synthesized where phosphorescent metal tags replace conventional nucleobases. Two new synthetic protocols join cyclometalated iridium(III) and platinum(II) to deoxyribose, creating emitting agents that monitor the biochemical reactions of nucleoside metabolism. The comprehensive studies proposed here will characterize these unique compounds at the molecular (ground- and excited-state methods), cellular (transport and imaging studies), and organismal (animal imaging studies) levels. The work will support student training in the Gray and Berdis research laboratories, whose members include participants from several underrepresented groups. The use of cyclometalated bioprobes is being incorporated into undergraduate and graduate-level Bioinorganic Chemistry courses in the Department of Chemistry at Case Western Reserve University.

1066107灰色核苷及其相应的三磷酸盐是正常细胞功能所需的基本构件。一个代表性不足的研究领域是开发和应用发射生物传感器来监测核苷在活细胞中的运输和代谢。为了实现这一目标，将在磷光金属标记取代传统碱基的情况下合成核苷。两种新的合成方案将环金属Ir(III)和铂(II)连接到脱氧核糖，创建了监测核苷代谢生化反应的发射剂。这里提出的综合研究将在分子(基态和激发态方法)、细胞(运输和成像研究)和生物体(动物成像研究)水平上表征这些独特的化合物。这项工作将支持格雷和伯迪斯研究实验室的学生培训，这些实验室的成员包括几个代表性不足的团体的参与者。凯斯西储大学化学系的本科生和研究生生物无机化学课程正在纳入环金属生物探针的使用。