Novel Biophotonic Probes to Monitor Cellular Metabolism of Nucleosides

1066107 Gray核苷及其相应的三磷酸是正常细胞功能所需的基本结构单元。一个代表性不足的研究领域是开发和应用发射生物传感器来监测活细胞中的核苷转运和代谢。为了实现这一目标，将合成核苷，其中磷光金属标签取代传统的核碱基。两种新的合成方法将环化铱（III）和铂（II）与脱氧核糖结合，创造出监测核苷代谢生化反应的发射剂。这里提出的全面研究将在分子（基态和激发态方法），细胞（运输和成像研究）和生物体（动物成像研究）水平上表征这些独特的化合物。这项工作将支持格雷和伯迪斯研究实验室的学生培训，其成员包括来自几个代表性不足的群体的参与者。环化生物探针的使用被纳入凯斯西储大学化学系的本科生和研究生生物无机化学课程。

1066107GrayNucleosides and their corresponding triphosphates are essential building blocks needed for proper cellular function. An underrepresented area of study is the development and application of emissive biosensors to monitor nucleoside transport and metabolism in living cells. Toward this goal, nucleosides will be synthesized where phosphorescent metal tags replace conventional nucleobases. Two new synthetic protocols join cyclometalated iridium(III) and platinum(II) to deoxyribose, creating emitting agents that monitor the biochemical reactions of nucleoside metabolism. The comprehensive studies proposed here will characterize these unique compounds at the molecular (ground- and excited-state methods), cellular (transport and imaging studies), and organismal (animal imaging studies) levels. The work will support student training in the Gray and Berdis research laboratories, whose members include participants from several underrepresented groups. The use of cyclometalated bioprobes is being incorporated into undergraduate and graduate-level Bioinorganic Chemistry courses in the Department of Chemistry at Case Western Reserve University.