Continuous Ethanol Fermentation and Recovery Using an Improved Zeolite Membrane Bioreactor

使用改进的沸石膜生物反应器连续乙醇发酵和回收

• 批准号: 1067684
• 负责人: David Nielsen
• 金额: $ 36.24万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-04-01 至 2015-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1067684&HistoricalAwards=false
• 关键词: Continuous; Ethanol; Fermentation; Recovery; Using

PI: Nielsen, David Institution: Arizona State University Proposal Number: 1067684Title: Continuous Ethanol Fermentation and Recovery using an ImprovedZeolite Membrane BioreactorThis research aims to develop a lab-scale bioprocess which integrates fermentation with zeolite membrane pervaporation for the high-rate, high-purity, and continuous production of biofuel ethanol. To support this objective, the research is composed of three principal aims: 1) the synthesis and development of improved, aluminum-free hydrophobic zeolite membranes with both elevated ethanol permselectivity and flux, 2) characterization of non-ideal physical/biological phenomena occurring at the membrane surface, and 3) the design, development, and characterization of novel bioprocesses incorporating zeolite membrane pervaporation as a means of economical, in situ product recovery.Intellectual Merit: The first principal aim focuses on the synthesis of high quality, aluminum-free silicalite membranes on chemically inert porous zirconia supports. These new membranes, which will be fabricated in both disk and tubular geometries, are expected to demonstrate much higher fluxes and ethanol/water selectivity than existing polymer and zeolite membranes. The second important aim is to provide fundamental insights into the mechanistic nature of non-ideal, physical and/or biological interactions occurring upon zeolite surfaces, as well as their resultant effect on membrane properties and impact on separation performance. These studies will help to develop a mechanistic understanding of biofouling (by both proteins and whole cells) as a function of relevant process conditions and biocatalyst genotype. The final aim is to design, develop, and characterize a scalable and integrated bioprocess for the continuous production and recovery of ethanol from yeast fermentations using zeolite membrane pervaporation. To support sustained and high-level performance, operating policies and fouling reduction strategies, to include in situ regeneration by membrane backflushing, will be developed and optimized.Broader Impact: This study will enable developments in separation science and bioprocess design, supporting the development of sustainable biofuels. The concept of aluminum-free, silicalite membrane preparation can ultimately be translated to the synthesis of other zeolite membranes to enable greater material compatibility and higher performance for other separation applications. Newly synthesized tubular zeolite membranes and their synthesis protocols could find other applications for separation of gas and liquid mixtures of industrial importance, such as refinery gas of hydrocarbon/hydrogen and other alcohol/water mixtures. The development of a bioprocess enabling the selective separation of inhibitory products by zeolite membrane pervaporation could have broader applications for the production of additional biochemicals and emerging, next generation biofuels. The characterization of physical/biological interactions at zeolite surfaces could be valuable for assessing the future prospects of zeolite materials in fields ranging from bioenergy production to biomedical engineering. The recruited personnel and dual-use equipment resources made possible through this study will enable the development of biologically-based laboratory modules to enrich the educational experiences of undergraduates.

PI：Nielsen，David研究所：亚利桑那州立大学提案编号：1067684标题：使用改进的沸石膜生物反应器进行乙醇的连续发酵和回收本研究旨在开发一种将发酵和沸石膜渗透汽化相结合的实验室规模的生物工艺，以实现高速率、高纯度和连续生产生物燃料乙醇。为了支持这一目标，这项研究包括三个主要目标：1)合成和开发具有更高的乙醇渗透选择性和通量的改进型无铝疏水沸石膜，2)表征膜表面发生的非理想物理/生物现象，以及3)设计、开发和表征新型生物过程，将分子筛膜渗透汽化作为一种经济、原位产品回收的手段。智力优势：第一个主要目标是在化学惰性多孔氧化锆载体上合成高质量的无铝硅沸石膜。这些新型膜将以盘状和管状两种几何形状制备，预计将比现有的聚合物和沸石膜表现出更高的通量和乙醇/水的选择性。第二个重要目标是对沸石表面发生的非理想、物理和/或生物相互作用的机理及其对膜性能的影响和对分离性能的影响提供基本见解。这些研究将有助于从机理上理解生物污垢(由蛋白质和整个细胞)作为相关工艺条件和生物催化剂基因型的函数。最终目标是设计、开发和表征一种可扩展的集成生物工艺，用于使用沸石膜渗透汽化从酵母发酵中连续生产和回收乙醇。为了支持持续和高水平的性能，将制定和优化运行政策和减少污染的战略，包括通过膜反冲洗进行现场再生。广泛影响：这项研究将促进分离科学和生物工艺设计的发展，支持可持续生物燃料的开发。无铝硅沸石膜制备的概念最终可以转化为合成其他沸石膜，以实现更好的材料兼容性和更高的性能，用于其他分离应用。新合成的管状沸石膜及其合成方案可用于分离工业上重要的气体和液体混合物，如烃/氢和其他醇/水混合物的炼油厂气体。开发一种能够通过沸石膜渗透汽化选择性分离抑制产物的生物工艺可以在生产更多的生物化学品和新兴的下一代生物燃料方面有更广泛的应用。沸石表面物理/生物相互作用的表征对于评估沸石材料在从生物能源生产到生物医学工程等领域的未来前景具有重要意义。通过这项研究招募的人员和两用设备资源将使生物实验室模块的开发成为可能，以丰富本科生的教育经验。