Molecular mechanisms of toxin-induced susceptibility and plant immunity-Functional diversification within the microbial NLP superfamily

毒素诱导敏感性和植物免疫的分子机制-微生物NLP超家族内的功能多样化

• 批准号: 200986420
• 负责人: Professor Dr. Thorsten Nürnberger
• 金额: --
• 依托单位: Abteilung für Pflanzenbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200986420?language=en
• 关键词: Molecular; mechanisms; toxin; induced; susceptibility

Host leaf necrosis and ethylene-inducing peptide 1-like proteins (NLP) are produced by plant pathogenic species from three microbial lineages; bacteria, fungi and oomycetes. Many NLPs are pore-forming toxins that facilitate infection of eudicot plants upon binding to eudicot-specific glycosphingolipid toxin receptors. Identification of NLP proteins lacking cytolytic activities suggests functional diversification within this microbial superfamily. Expression patterns of genes encoding non-cytolytic NLPs (ncNLPs) and tertiary structure similarities of ncNLPs with fungal lectins suggest that these proteins may play roles in host attachment at early phases of host infection. However, no evidence is available for a role of ncNLPs as microbial virulence factors. Here, we propose to conduct a comprehensive analysis of the putative disease-promoting activities of twelve ncNLPs from four different microbial species by transient expression in Nicotiana benthamiana and subsequent infection with Phytophthora infestans. We further attempt to study a proposed role of ncNLPs as lectin-like molecules during early host infection, and to identify host target structures for ncNLPs. In addition, we devise a strategy to control parasitic plant infestation in solanaceous plants by conditional, infection site-specific expression of a cytolytic NLP.

寄主叶片坏死和乙烯诱导肽1样蛋白（NLP）是由三种微生物谱系的植物病原菌产生的；细菌，真菌和卵菌。许多nlp是成孔毒素，通过与苦苣苔特异性糖鞘脂毒素受体结合，促进苦苣苔植物的感染。鉴定缺乏细胞分解活性的NLP蛋白表明在这个微生物超家族中功能多样化。编码非细胞溶解性nlp （ncnlp）的基因表达模式以及ncnlp与真菌凝集素的三级结构相似性表明，这些蛋白可能在宿主感染早期的宿主附着中发挥作用。然而，没有证据表明ncnlp作为微生物毒力因子的作用。在这里，我们提议通过在本菌中短暂表达并随后感染疫霉菌，对来自4种不同微生物种的12种ncnlp的推定促病活性进行全面分析。我们进一步尝试研究ncNLPs作为凝集素样分子在早期宿主感染中的作用，并确定ncNLPs的宿主靶结构。此外，我们设计了一种策略来控制寄生植物侵染在茄科植物的条件，感染位点特异性表达细胞溶解NLP。