Functional analysis of Arabidopsis leucine-rich repeat receptor-like kinases (LRR-RLK) with dual roles in plant immunity and development

在植物免疫和发育中具有双重作用的拟南芥富含亮氨酸重复受体样激酶（LRR-RLK）的功能分析

• 批准号: 49886109
• 负责人: Professor Dr. Thorsten Nürnberger
• 金额: --
• 依托单位: Abteilung für Pflanzenbiochemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/49886109?language=en
• 关键词: Functional; analysis; Arabidopsis; leucine; rich

Receptor-like kinases (RLK) serve various roles in plants. The vast number of Arabidopsis leucine-rich repeat RLK (LRR-RLK) genes and the fact that a few of the encoded proteins are implicated in plant immunity suggest that various LRR-RLKs play predominant roles in plant-pathogen interactions. As contributors to the AtGenExpress Initiative we have conducted gene expression profiling experiments on microbe-infected plants. Our studies revealed 49 (out of 228) LRR-RLK genes whose expression was increased upon infection. Some of the encoded proteins were shown to contribute to plant immunity. BAK1 (BRI1-Associated Kinase1) regulates the containment of microbial infection-induced necrosis and resistance against necrotrophic fungi. Importantly, impaired immunity in bak1 mutants is independent of the phytohormone, brassinolide (BL). Thus, BAK1 appears to serve BL-dependent and independent functions in development and immunity. Likewise, PSKR1 (pentasulfokine receptor 1) represents another LRR-RLK with dual roles in plant immunity and development. Dual function proteins are considered nodes in partially overlapping biological programs whose regulation is often unknown. We will therefore address what determines specificity of the two proteins in development and immunity. The proposed work aims at identification and characterization of proteins interacting with BAK1 or PSKR1, respectively. Our research will intensify established links to US and German Partners who study development-associated LRR-RLKs.

受体样激酶（RLK）在植物中发挥着多种作用。大量的拟南芥富含亮氨酸的重复序列RLK（LRR-RLK）基因和事实上，一些编码的蛋白质涉及植物免疫表明，各种LRR-RLK在植物-病原体相互作用中发挥主导作用。作为AtGenExpress倡议的贡献者，我们对受微生物感染的植物进行了基因表达谱分析实验。我们的研究揭示了49个（228个）LRR-RLK基因，其表达在感染后增加。一些编码的蛋白质被证明有助于植物免疫。BAK 1（BRI 1-Associated Kinase 1）调节微生物感染诱导的坏死的遏制和对坏死营养型真菌的抗性。重要的是，bak 1突变体的免疫功能受损是独立的植物激素，油菜素（BL）。因此，BAK 1似乎在发育和免疫中发挥BL依赖性和独立性功能。同样，PSKR 1（pentasulfokine receptor 1）代表另一种LRR-RLK，在植物免疫和发育中具有双重作用。双功能蛋白被认为是部分重叠的生物学程序中的节点，其调节通常是未知的。因此，我们将讨论是什么决定了这两种蛋白质在发育和免疫中的特异性。所提出的工作旨在分别鉴定和表征与BAK 1或PSKR 1相互作用的蛋白质。我们的研究将加强与美国和德国合作伙伴的联系，他们研究与发育相关的LRR-RLKs。