Further Investigations into Aleut Subsistence: Analysis of Bone Apatite for Carbon and Oxygen, Bone Collagen for Sulfur and Individual Amino Acid Isotope Chemistry

拟议的项目是先前研究的后续和扩展，使用稳定同位素分析和古DNA，利用来自人类和非人类动物遗骸的样本，从东阿留申群岛的查卢卡中登遗址、Shiprok和Kagamil洞穴四个地点讨论种群历史。最初的项目提出了关于古生代和古生代之间生物学差异的假设。然后呢？新阿留申，？最初是使用颅骨测量学和颅骨形态来定义的，并得出结论，差异并不反映先前假设的大规模人口更替。科林？S先前的分析结果表明，不同地区的历史人口对陆地和海洋生存资源的使用存在差异。该项目将扩大稳定同位素的组合，特别是硫，以分离食物分类群，碳同位素与骨磷灰石，氧同位素作为温度水平的替代，以及化合物特有的氮，以分离特定的氨基酸。对这些不同同位素的分析将使研究人员能够更密切地确定历史种群中代表的个体的饮食，并提供更具体的证据，证明同一种群之间存在饮食分离，而不是像以前认为的那样，代表两个不同种群的骨骼样本。

The proposed project is a follow-up and expansion of preious research using stable isotope analysis and paleo-DNA to discuss the population history from four locations, the Chaluka midden site, Shiprock, and Kagamil caves, in the Eastern Aleutians, using samples from human and non-human faunal remains. The original project addressed hypotheses concerning the biological difference between ?Paleo? and ?Neo-Aleuts,? originally defined using craniometrics and cranial morphology and concluded that the differences did not reflect wholesale population replacement as had previously been hypothesized. The results from Coltrain?s previous analysis suggested that the historic populations in different localities differentially used terrestrial and marine subsistence resources. This project would expand the suite of stable isotopes, specifically sulfur to separate food taxa, carbon isotopes from bone apatite, oxygen isotopes as a proxy for temperature levels, and compound-specific nitrogen to separate specific amino acids. Analysis of these various isotopes would allow the researcher to more closely identify the diets of the individuals represented in the historic populations and provide more specific evidence for a separation of diet between the same population, rather than the skeletal sample representing two separate populations as previously thought.