Further Investigations into Aleut Subsistence: Analysis of Bone Apatite for Carbon and Oxygen, Bone Collagen for Sulfur and Individual Amino Acid Isotope Chemistry

该计划是先前研究的后续和扩展，使用稳定同位素分析和古dna来讨论四个地点的人口历史，Chaluka midden遗址，Shiprock和Kagamil洞穴，在东阿留申群岛，使用人类和非人类动物遗骸的样本。最初的项目提出了关于生物差异的假设？穿越?和Neo-Aleuts, ?最初是用颅测量学和颅形态学来定义的，并得出结论，这些差异并没有像之前假设的那样反映出人口的大规模更替。Coltrain的结果？前人的分析表明，不同地区的历史种群对陆地和海洋生存资源的利用存在差异。该项目将扩大稳定同位素的范围，特别是用于分离食物类群的硫，用于分离骨磷灰石的碳同位素，用于代表温度水平的氧同位素，以及用于分离特定氨基酸的化合物特定氮。对这些不同同位素的分析将使研究人员能够更密切地识别历史种群中所代表的个体的饮食，并为同一种群之间的饮食分离提供更具体的证据，而不是像之前认为的那样代表两个不同种群的骨骼样本。

The proposed project is a follow-up and expansion of preious research using stable isotope analysis and paleo-DNA to discuss the population history from four locations, the Chaluka midden site, Shiprock, and Kagamil caves, in the Eastern Aleutians, using samples from human and non-human faunal remains. The original project addressed hypotheses concerning the biological difference between ?Paleo? and ?Neo-Aleuts,? originally defined using craniometrics and cranial morphology and concluded that the differences did not reflect wholesale population replacement as had previously been hypothesized. The results from Coltrain?s previous analysis suggested that the historic populations in different localities differentially used terrestrial and marine subsistence resources. This project would expand the suite of stable isotopes, specifically sulfur to separate food taxa, carbon isotopes from bone apatite, oxygen isotopes as a proxy for temperature levels, and compound-specific nitrogen to separate specific amino acids. Analysis of these various isotopes would allow the researcher to more closely identify the diets of the individuals represented in the historic populations and provide more specific evidence for a separation of diet between the same population, rather than the skeletal sample representing two separate populations as previously thought.