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Dissertation Research: Multiple Paternity in P. antipodarum, a New Zealand Snail Species

论文研究：新西兰蜗牛种 P. antipodarum 的多重亲子关系

基本信息

批准号：
1110396
负责人：
Curtis Lively
金额：
$ 1.23万
依托单位：
Indiana University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2011
资助国家：
美国
起止时间：
2011-06-15 至 2013-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1110396&HistoricalAwards=false
关键词：
Dissertation Research Multiple Paternity P.

项目摘要

Genetic diversity is an important factor that assists in maintaining the health of a population. Mating behaviors may be one way for organisms to increase the genetic diversity of a population. For example, if a female mates with multiple males, her offspring will have greater genetic diversity than a female that mates with only one male. This project aims to test for multiple mating, and determine if it assists in increasing disease resistance in a freshwater snail from New Zealand. Molecular techniques including PCR and microsatellite analysis will be used to assess paternity. A laboratory experiment will be used to create single-father and multiple-father families, which will then be tested for disease resistance. Since multiple paternity has been found in one population, we expect to find multiple paternity in a second population. We also predict that multiple paternity will increase the chance that some offspring will be disease resistant. This research will contribute to many different areas of scientific research, including how genetics affect disease transmission, the evolution of reproductive behaviors, and generation of genetic diversity through mating decisions. Resulting research is intended to contribute to the scientific community through publishing and presenting results and also to education through integration of outcomes in undergraduate course work.

遗传多样性是帮助维持种群健康的一个重要因素。交配行为可能是生物体增加种群遗传多样性的一种方式。例如，如果一只雌性与多个雄性交配，它的后代将比只与一个雄性交配的雌性具有更大的遗传多样性。该项目旨在测试多重交配，并确定它是否有助于提高新西兰淡水蜗牛的抗病能力。包括PCR和微卫星分析在内的分子技术将用于鉴定亲子关系。一项实验室实验将用于创建单亲父亲和多父亲家庭，然后对这些家庭进行抗病测试。由于在一个种群中发现了多重父权，我们期望在第二个种群中发现多重父权。我们还预测，多父关系将增加一些后代抗病的机会。这项研究将有助于许多不同领域的科学研究，包括遗传如何影响疾病传播，生殖行为的进化，以及通过交配决定产生遗传多样性。结果研究旨在通过发表和展示结果为科学界做出贡献，并通过将结果整合到本科课程作业中为教育做出贡献。