RUI: Hyphenated Surface Plasmon Resonance Techniques for Enhanced Biomolecular Interaction Studies and Biomarker Detections

RUI：用于增强生物分子相互作用研究和生物标志物检测的联用表面等离子共振技术

• 批准号: 1112105
• 负责人: Feimeng Zhou
• 金额: $ 27万
• 依托单位: California State L A University Auxiliary Services Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1112105&HistoricalAwards=false
• 关键词: RUI; Hyphenated; Surface; Plasmon; Resonance

Professor Feimeng Zhou of California State L A University is supported by the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry to interface surface plasmon resonance (SPR) with various analytical and separation techniques and use the hyphenated analytical tools to study molecular binding events and to identify biomarkers of relevance to neurological disorders. Three related projects are proposed. Electrochemical flow-injection SPR will be used to determine ligand-receptor binding; imaging SPR will be used for high-throughput screening of potential inhibitors of beta-secretase, which is relevant to the pathology of Alzheimer's disease; and label-free SPR will be combined with size-exclusion chromatography for the separation and detection of Alzheimer's disease-related peptides and protein fragments. If successful, the proposed research will lead to the development of new hyphenated analytical tools which may positively impact the diagnosis and therapy of Alzheimer's disease and other neurological disorders. Undergraduate students in a minority serving institution will be trained in analytical instrumentation, device fabrication, surface chemistry, biosensor, and neurochemistry, and will be motivated to pursue advanced studies in chemistry.

加利福尼亚州L大学的周飞萌教授得到了化学系化学测量和成像计划的支持，将表面等离子体共振与各种分析和分离技术相结合，并使用联用的分析工具来研究分子结合事件和识别与神经系统疾病相关的生物标志物。提出了三个相关的项目。电化学流动注射SPR将用于确定配体与受体的结合；成像SPR将用于高通量筛选与阿尔茨海默病病理相关的β-分泌酶潜在抑制剂；无标记SPR将与尺寸排除层析相结合，用于分离和检测与阿尔茨海默病相关的多肽和蛋白质片段。如果成功，拟议的研究将导致开发新的联用分析工具，这可能会对阿尔茨海默病和其他神经疾病的诊断和治疗产生积极影响。少数族裔服务机构的本科生将接受分析仪器、设备制造、表面化学、生物传感器和神经化学方面的培训，并将受到激励，继续深造化学。