RUI: Hyphenated Surface Plasmon Resonance Techniques for Enhanced Biomolecular Interaction Studies and Biomarker Detections

美国加州州立洛杉矶大学化学测量与成像（CMI）项目的周飞梦教授在化学系的支持下，将表面等离子体共振（SPR）与各种分析和分离技术相结合，并使用连线分析工具研究分子结合事件并识别与神经系统疾病相关的生物标志物。提出了三个相关项目。电化学流动注射SPR将用于确定配体-受体结合；成像SPR将用于高通量筛选β -分泌酶的潜在抑制剂，这与阿尔茨海默病的病理有关；无标签SPR将与尺寸排除色谱相结合，用于阿尔茨海默病相关肽和蛋白质片段的分离和检测。如果成功，这项研究将导致新的联用分析工具的发展，这可能对阿尔茨海默病和其他神经系统疾病的诊断和治疗产生积极的影响。少数民族服务机构的本科生将接受分析仪器、设备制造、表面化学、生物传感器和神经化学方面的培训，并将被激励在化学方面继续深造。

Professor Feimeng Zhou of California State L A University is supported by the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry to interface surface plasmon resonance (SPR) with various analytical and separation techniques and use the hyphenated analytical tools to study molecular binding events and to identify biomarkers of relevance to neurological disorders. Three related projects are proposed. Electrochemical flow-injection SPR will be used to determine ligand-receptor binding; imaging SPR will be used for high-throughput screening of potential inhibitors of beta-secretase, which is relevant to the pathology of Alzheimer's disease; and label-free SPR will be combined with size-exclusion chromatography for the separation and detection of Alzheimer's disease-related peptides and protein fragments. If successful, the proposed research will lead to the development of new hyphenated analytical tools which may positively impact the diagnosis and therapy of Alzheimer's disease and other neurological disorders. Undergraduate students in a minority serving institution will be trained in analytical instrumentation, device fabrication, surface chemistry, biosensor, and neurochemistry, and will be motivated to pursue advanced studies in chemistry.