Collaborative Research: Advancing In-vivo Chemical Imaging by Merging Synchrotron-Based Infrared Data, Rapid Spectral Image Analysis, and Quantitative Chemometrics

Carol J. Hirschmugl和Roshan M.威斯康星大学密尔沃基分校的Dsouza和田纳西大学诺克斯维尔的Frank沃格特教授得到化学系化学测量和成像（CMI）计划的支持，开展一个合作项目，以推进体内化学成像和分析。这项研究将结合同步辐射为基础的红外数据获得的高光谱成像光谱仪（IRENI）在同步辐射中心在麦迪逊，WI与快速光谱图像分析，使用图形处理单元和定量化学计量学。该研究涉及活细胞的高分辨率成像，散射校正，复杂系统中浓度测量的化学计量学方法以及藻类生长研究的应用。如果成功，该项目将提高IRENI的性能，并使以前被认为过于复杂而无法处理的样品（如活细胞）的化学成像和分析成为可能。该项目将在化学和生物学方面产生广泛的影响。学生将在同步辐射中心接受最先进的红外成像仪器的培训。来自不同群体的本科生及其教师将通过现有的REU和RET计划参与该项目，并将举办研讨会，以使社区熟悉IRENI并扩大其技术用户群。

Professors Carol J. Hirschmugl and Roshan M. Dsouza of the University of Wisconsin-Milwaukee and Professor Frank Vogt of the University of Tennessee Knoxville are supported by the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry to conduct a collaborative project to advance in-vivo chemical imaging and analysis. The research will be conducted by combining synchrotron-based infrared data obtained with the hyperspectral imaging spectrometer (IRENI) at the Synchrotron Radiation Center at Madison, WI with rapid spectral image analysis using graphics processing units and quantitative chemometrics. The research involves high resolution imaging of live cells, scattering corrections, chemometric methods for concentration measurement in complex systems and application to algae growth studies. If successful the project will improve the performance of IRENI, the premium facility of its kind, and enable the chemical imaging and analysis of samples that were previously considered to be too complex to handle, such as live cells. The project will have a broad impact in chemistry and biology. Students will be trained on a state-of-the-art infrared imaging instrument in a Synchrotron Radiation Center. Undergraduate students from diverse groups and their teachers will be involved in the project through existing REU and RET programs, and workshops will be conducted to familiarize the community with IRENI and to broaden its technical user base.