Advancing 3D Chemical Imaging: FTIR Spectro-microtomography, FTIR Spectro-microlaminography and Hyperspectral Data Analysis

有了这个奖项，化学部的化学测量和成像计划正在资助威斯康星大学密尔沃基分校的Carol Hirschmugl和Sarah Patch教授，以推进生物相关材料的三维（3D）化学成像。为了应对这一挑战，对红外光谱显微层析成像的修改将与高光谱数据分析工具相结合，以评估扩展的二维样本。威斯康星大学密尔沃基分校，卫斯理大学和挪威生命科学大学之间的国际合作结合了新颖的仪器，前沿图像重建算法和物理精确建模，以产生化学敏感的三维图像。预计该项目将在具有国际层面的协作环境中提供多学科的学生培训。该提案的总体目标是实现傅里叶变换红外（FTIR）光谱显微断层扫描（SmT）的样品，在两个维度上扩展，并实现有限的角度重建算法和优化光谱化学分析方法，以提高光谱和图像质量。这一建议的基本原理是，改进的新型化学成像技术结合先进的数据分析的数据可能能够提供完整的，（生物）化学复杂系统的新观点。该项目的重点是生物相关样品（例如，组织，扩展细胞培养物，生物膜）或生物能源材料（例如，藻类，木材）的三维成像。 FTIR-SmT旨在通过采集有机材料的丰富红外吸收光谱来产生高对比度结果。这种检测完整样品的化学分布的能力可能为生物分析化学中的全新方法提供基础，远远超出这些研究。

With this award, the Chemical Measurement and Imaging Program of the Division of Chemistry is funding Professors Carol Hirschmugl and Sarah Patch of The University of Wisconsin-Milwaukee to advance three-dimensional (3D) chemical imaging for biologically relevant materials. To meet this challenge, modifications to infrared spectral micro- tomography are to be combined with hyperspectral data analysis tools to evaluate extended two-dimensional samples. The international collaboration between The University of Wisconsin-Milwaukee, Wesleyan University, and the Norwegian University of Life Sciences combines novel instrumentation, forefront image reconstruction algorithms, and physically accurate modeling to produce chemically sensitive 3-dimensional images. The project is expected to provide multi-disciplinary disciplinary student training in a collaborative environment with international dimensions. The overall objective of this proposal is to implement Fourier transform infrared (FTIR) spectro-micro-tomography (SmT) for samples that are extended in two dimensions and to implement limited angle reconstruction algorithms and optimize spectrochemical analysis methods to enhance spectral and image quality. The rationale for this proposal is that data from improved novel chemical imaging techniques combined with advanced data analysis may be able to provide new views of of intact, (bio)chemically complex systems. This project focuses on three-dimensional imaging of biologically relevant samples (e.g, tissues, extended cell cultures, biofilms) or to materials for bioenergy (e.g, algae, wood). FTIR-SmT aims to generate high contrast results through the acquisition of rich IR absorption spectra for organic materials. Such a capability for sensing chemical distributions for intact samples may provide the foundation for fundamentally new approaches in bioanalytical chemistry, well beyond these studies.