Advancing 3D Chemical Imaging: FTIR Spectro-microtomography, FTIR Spectro-microlaminography and Hyperspectral Data Analysis

化学系化学测量和成像项目将通过该奖项资助威斯康星大学密尔沃基分校的Carol Hirschmugl和Sarah Patch教授，以推进生物相关材料的三维（3D）化学成像。为了应对这一挑战，红外光谱微层析成像的改进将与高光谱数据分析工具相结合，以评估扩展的二维样品。威斯康星大学密尔沃基分校、卫斯理大学和挪威生命科学大学之间的国际合作结合了新型仪器、前沿图像重建算法和物理精确建模，以产生化学敏感的三维图像。该项目预计将在具有国际规模的协作环境中提供多学科的学生培训。本提案的总体目标是对二维扩展的样品实施傅里叶变换红外（FTIR）光谱微层析成像（SmT），并实施有限角度重建算法和优化光谱化学分析方法，以提高光谱和图像质量。该提议的基本原理是，来自改进的新型化学成像技术的数据与先进的数据分析相结合，可能能够为完整的（生物）化学复杂系统提供新的视角。该项目侧重于生物相关样品（如组织、扩展细胞培养物、生物膜）或生物能源材料（如藻类、木材）的三维成像。FTIR-SmT旨在通过获取有机材料丰富的红外吸收光谱来产生高对比度的结果。这种感知完整样品化学分布的能力可能为生物分析化学的新方法提供基础，远远超出这些研究。

With this award, the Chemical Measurement and Imaging Program of the Division of Chemistry is funding Professors Carol Hirschmugl and Sarah Patch of The University of Wisconsin-Milwaukee to advance three-dimensional (3D) chemical imaging for biologically relevant materials. To meet this challenge, modifications to infrared spectral micro- tomography are to be combined with hyperspectral data analysis tools to evaluate extended two-dimensional samples. The international collaboration between The University of Wisconsin-Milwaukee, Wesleyan University, and the Norwegian University of Life Sciences combines novel instrumentation, forefront image reconstruction algorithms, and physically accurate modeling to produce chemically sensitive 3-dimensional images. The project is expected to provide multi-disciplinary disciplinary student training in a collaborative environment with international dimensions. The overall objective of this proposal is to implement Fourier transform infrared (FTIR) spectro-micro-tomography (SmT) for samples that are extended in two dimensions and to implement limited angle reconstruction algorithms and optimize spectrochemical analysis methods to enhance spectral and image quality. The rationale for this proposal is that data from improved novel chemical imaging techniques combined with advanced data analysis may be able to provide new views of of intact, (bio)chemically complex systems. This project focuses on three-dimensional imaging of biologically relevant samples (e.g, tissues, extended cell cultures, biofilms) or to materials for bioenergy (e.g, algae, wood). FTIR-SmT aims to generate high contrast results through the acquisition of rich IR absorption spectra for organic materials. Such a capability for sensing chemical distributions for intact samples may provide the foundation for fundamentally new approaches in bioanalytical chemistry, well beyond these studies.