MRI: Development of Synchrotron Based Infrared Microspectroscopy with a Multi-Element Detector

技术摘要威斯康星大学密尔沃基分校将设计一种中红外(IR)光束线IRMSI-MED，可提取400小时。X 25 Vert.来自同步加速器弯曲磁铁的mrads2，将被开发为均匀照射配备多元素探测器(MED)的商用红外显微镜。这将提供在几分钟内获得衍射有限(或更好)分辨率为35平方毫米的区域的化学图像的机会。最初的研究项目包括研究活细胞(浮游植物)、真菌和细菌-矿物相互作用的动力学。该项目有可能影响广泛的研究领域，从软物质凝聚物理、纳米科学、生物、化学、兽医科学、工程、环境科学和地质学。这一发展将影响研究生、约旦SESAME计划的国际合作者、高中生、REU学生和将被鼓励使用该设施的SRC人员计划的参与者的研究，并将举办旨在教育新用户的研讨会。从最早的光学显微镜实验开始，研究人员已经检查了微生物和其他微观动植物的外观；他们努力识别明显的各种细胞器和亚细胞结构，帮助它们推断它们的生物功能。除了这些结构的视觉外观外，了解它们的化学组成将为了解这些亚细胞结构如何在活细胞中发挥作用提供极大的洞察力。此外，跟踪它们化学组成的变化将使科学家能够了解生物体对不断变化的环境条件的反应。具有多个平行探测通道的化学灵敏红外显微镜的开发将极大地扩展检查这种生物结构并在几分钟内跟踪它们的变化的能力。该显微镜将向广泛学科的用户提供(例如，软物质凝聚物理、纳米科学、生物、化学、兽医科学、工程学、环境科学和地质学)，为更广泛的科学界提供一种新的跨学科工具。许多研究生将参与到这些研究工作中来。此外，将鼓励高中生、REU学生和人员计划的参与者使用该设施，并将举办旨在教育新用户的研讨会。

Technical AbstractUniversity of Wisconsin-Milwaukee will design a mid-infrared (IR) beamline, IRMSI-MED, extracting 400 hor. x 25 vert. mrads2 from a synchrotron bending magnet, that will be developed to homogeneously illuminate a commercial IR microscope equipped with a multi-element detector (MED). This will provide the opportunity to obtain chemical images with diffraction-limited (or better) resolution of 35 mm2 square areas in minutes. Initial research projects include examining kinetics of living cells (phytoplankton), fungi and bacteria-mineral interactions. This project has a potential to impact a wide variety of research areas ranging from soft matter condensed physics, nanoscience, biology, chemistry, veterinary science, engineering, environmental science and geology. This development will impact the research of graduate students, international collaborators from the SESAME program in Jordan, high school students, REU students and participants in the PEOPLE program at the SRC that will be encouraged to use the facility, and there will be workshops designed to educate new users. Lay AbstractFrom the earliest experiments with optical microscopes, researchers have examined the appearance of microbes and other microscopic plants and animals; they have strived to identify the various organelles and sub-cellular structures evident, helping them to infer their biological function. Beyond the visual appearance of these structures, a knowledge of their chemical makeup would provide great insight into how these sub-cellular structures function in a living cell. Moreover, tracking the changes in their chemical makeup would allow scientists to understand the organism's response to changing environmental conditions. The development of a chemically sensitive infrared microscope with multiple, parallel detection channels will greatly expand the ability to examine such biological structures, and to track their changes over minutes. The microscope will be available for users across a wide array of disciplines (e.g. soft matter condensed physics, nanoscience, biology, chemistry, veterinary science, engineering, environmental science and geology), providing a new interdisciplinary tool to the broader scientific community. Many graduate students will be involved in these research efforts. In addition, high school students, REU students and participants in the PEOPLE program will be encouraged to use the facility, and there will be workshops designed to educate new users.