MRI: Acquisition of a seed x-ray machine

MRI：购买种子 X 射线机

• 批准号: 1125997
• 负责人: Kayri Havens
• 金额: $ 13.66万
• 依托单位: Chicago Horticultural Society
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1125997&HistoricalAwards=false
• 关键词: MRI; Acquisition; seed; ray; machine

The Chicago Botanic Garden will acquire a seed x-ray machine to advance research in its seed/reproductive biology laboratory. A center for processing, testing, storing, genetically analyzing, and germinating seeds, this laboratory supports research and conservation efforts, as well as educational programs spanning from middle school through PhD programs. The Garden's seed-related research falls into three broad categories: ecological and restoration research, research on ex situ (off site) conservation methods, and horticultural research. Addition of an x-ray cabinet will serve research programs in all three areas by greatly expanding the lab's analytical capabilities. The Garden's studies encompass the biology of small, fragmented populations; the genetics and demography of both rare and invasive plant species; plant responses to climate change and to ecosystem management; and spatial and temporal patterns of molecular genetic diversity in populations. The Garden is also conducting restoration genetics studies that address seed transfer zone delineation and genetic erosion during seed storage and the process of ecological restoration. The Garden is a founding partner in Seeds of Success, the US native seed banking program, and partners with the Royal Botanic Gardens, Kew and USDA National Center for Genetic Resources Preservation to provide redundant storage for its Dixon National Tallgrass Prairie Seed Bank which currently holds over 1600 wild-collected, native species and will ultimately hold samples from multiple populations of the entire Midwestern flora (approximately 3000 species). The Dixon seed bank also holds multiple accessions of the twelve globally rare species we curate for the Center for Plant Conservation (CPC). Additionally, the Garden conducts research on perennial plant breeding to develop landscape plants adapted to the conditions of the Midwestern U.S. Research to assess the invasive potential of plant introductions to the Chicago region is also conducted. To date studies have examined fecundity and seed viability of potentially invasive ornamental taxa, having implications for the nursery industry and home gardeners.The acquisition of additional equipment for the seed/reproductive biology laboratory will result in an increase of the number and diversity of seed studies undertaken which have applications beyond the Garden. Seed banking of globally imperiled plant taxa and of the entire flora of the tallgrass prairie ecosystem benefits society at large. The seed bank serves as a genetic safety net, preventing extinction and mitigating against genetic erosion in wild populations. Several studies at the Garden address restoration genetics and the development of seed transfer zones. Federal, state and regional agency partners including the Bureau of Land Management, the USDA Forest Service, and Chicago Wilderness are implementing the seed transfer guidelines that the Garden develops in a variety of large-scale restoration projects. The results of our research will be disseminated not only to the scientific, conservation, and horticultural communities, but also to the general public through our on-site scientific interpretation (reaching over 900,000 visitors per year), membership publications (sent to over 48,000 members) and Web site that receives over 19 million hits per year.The Garden has a comprehensive education program in plant conservation biology, serving middle and high school students in our College and Science First programs and hosting several internships including an NSF-REU (Research Experiences for Undergraduates) program. The Garden also serves graduate students in a Master's program in partnership with Northwestern University and Ph.D. programs with both Northwestern University and University of Illinois Chicago. Professional training in conservation through workshops, short courses and continuing education classes are also offered. Perhaps most importantly, the seed x-ray equipment will fill a national infrastructure need. Currently, similar laboratories are primarily limited to U.S.D.A facilities in Fort Collins, CO, Beltsville, MD, Columbus, OH and a few other germplasm repository sites. The U.S.D.A. facilities focus almost exclusively on crops, crop relatives and ornamental plants. The Garden's proposed seed storage and research facility will build much-needed capacity in Chicago and the upper Midwest to conduct seed related research and germplasm preservation for native plants, including those that are rare.

芝加哥植物园将获得一台种子x光机，以推进其种子/生殖生物学实验室的研究。作为处理、测试、储存、基因分析和发芽种子的中心，该实验室支持研究和保护工作，以及从中学到博士课程的教育项目。与植物园种子相关的研究分为三大类：生态和修复研究、迁地保护方法研究和园艺研究。增加的x射线柜将通过极大地扩展实验室的分析能力，服务于所有三个领域的研究项目。“花园”的研究涵盖了小而分散的种群的生物学；珍稀和外来入侵植物的遗传和人口统计学研究植物对气候变化和生态系统管理的响应；以及种群分子遗传多样性的时空格局。该园还开展了恢复遗传学研究，以解决种子储存和生态恢复过程中的种子转移区划定和遗传侵蚀问题。该花园是美国本土种子银行项目“成功种子”的创始合作伙伴，并与皇家植物园、邱园和美国农业部国家遗传资源保护中心合作，为其迪克森国家高草草原种子银行提供冗余存储，该种子银行目前拥有超过1600种野生采集的本地物种，最终将保存整个中西部植物群的多个种群样本（约3000种）。Dixon种子库还拥有我们为植物保护中心（CPC）策划的12种全球稀有物种的多个条目。此外，公园还开展了多年生植物育种研究，以开发适应美国中西部条件的景观植物。还开展了植物引进芝加哥地区的入侵潜力评估研究。迄今为止，研究已经检查了潜在入侵观赏类群的繁殖力和种子活力，对苗圃工业和家庭园丁有影响。为种子/生殖生物学实验室购置了更多设备后，将增加种子研究的数量和多样性，这些研究的应用范围将超出菜园。全球濒危植物分类群和高草草原生态系统的整个植物区系的种子库有益于整个社会。种子库是一个遗传安全网，防止野生种群的灭绝和减轻遗传侵蚀。在该园进行的几项研究涉及恢复遗传学和种子转移区的发展。包括土地管理局、美国农业部林务局和芝加哥荒野局在内的联邦、州和地区机构合作伙伴正在实施公园在各种大规模恢复项目中制定的种子转移指南。我们的研究成果不仅将传播给科学、保护和园艺界，而且还将通过我们的现场科学解释（每年吸引90多万名参观者）、会员出版物（发送给4.8万多名会员）和每年访问量超过1900万次的网站传播给公众。植物园有一个全面的植物保护生物学教育项目，为我们的大学和科学第一项目的中学生和高中生提供服务，并举办了包括NSF-REU（本科生研究经历）项目在内的几个实习项目。该花园还与西北大学合作，为研究生提供硕士课程，并与西北大学和伊利诺伊大学芝加哥分校合作提供博士课程。此外，我们还提供工作坊、短期课程和继续教育课程，为保育工作提供专业培训。也许最重要的是，种子x射线设备将满足国家基础设施的需求。目前，类似的实验室主要局限于位于科罗拉多州柯林斯堡、马里兰州贝尔茨维尔、俄亥俄州哥伦布市和其他一些种质资源库站点的美国农业部设施。美国农业部的设施几乎完全专注于农作物、农作物近亲和观赏植物。花园计划中的种子储存和研究设施将在芝加哥和中西部上游地区建立急需的能力，以进行种子相关的研究和本地植物的种质保存，包括那些稀有植物。

项目成果

Fire influences reproductive outcomes by modifying flowering phenology and mate‐availability

火灾通过改变开花物候和交配可用性来影响繁殖结果

• DOI: 10.1111/nph.17923
• 发表时间: 2022
• 期刊: New Phytologist
• 影响因子: 9.4
• 作者: Richardson, Lea K.;Wagenius, Stuart
• 通讯作者: Wagenius, Stuart

Fire effects on plant reproductive fitness vary among individuals reflecting pollination‐dependent mechanisms

火灾对植物繁殖适应性的影响因个体而异，反映了授粉依赖机制

• DOI: 10.1002/ajb2.16160
• 发表时间: 2023
• 期刊: American Journal of Botany
• 影响因子: 3
• 作者: Richardson, Lea K.;Beck, Jared;Eck, Daniel J.;Shaw, Ruth;Wagenius, Stuart
• 通讯作者: Wagenius, Stuart