Structural and functional examinations of nucleotide-dependent membrane scaffolds (A12)

Eps15同源结构域蛋白（EHDs）是动力蛋白相关的atp酶，其在膜上寡聚形成atp依赖性支架。在过去的资助期内，我的小组利用x射线晶体学研究了ehd的活化机制。在未来，我们将结合生化、电子和超分辨率光学显微镜的方法，在结构水平上探索ehd支架如何在膜上组装，它们如何与伴侣蛋白相互作用，以及它们如何整合到细胞环境中，在膜运输中执行特定功能。

Eps15 homology domain containing proteins (EHDs) are dynamin-related ATPases that oligomerize on membranes to form ATP-dependent scaffolds. In the past funding period, my group has used X-ray crystallography to study the activation mechanism of EHDs. In the future, we will combine biochemical, electron and superresolution light microscopy approaches to explore on a structural level of how EHDs scaffolds assemble on membranes, how they interact with their partner proteins and how they are integrated into their cellular environment to execute specific functions in membrane trafficking.