A two-photon ophthalmoscope for human retinal imaging and functional testing

用于人类视网膜成像和功能测试的双光子检眼镜

• 批准号: 9059094
• 负责人: Krzysztof Palczewski
• 金额: $ 69.95万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-05-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9059094
• 关键词: Address; Affect; Age related macular degeneration; American; Americas; Area; Biochemical; Biochemistry; Cell Survival; Chills; Clinical; Clinical assessments; Collaborations; Computer software; Custom; Data; Dependence; Detection; Development; Diagnosis; Diagnostic; Diagnostic Imaging; Disease; Early Diagnosis; Electronics; Electrophysiology (science); Examinations and Diagnoses; Eye; Family suidae; Financial compensation; Fluorescence; Foundations; Functional Imaging; Funding; Genetically Engineered Mouse; Goals; Health; Human; Image; Imagery; Imaging technology; Individual; Institutes; Investigational Therapies; Knowledge; Lasers; Lesion; Light; Lighting; Mammals; Measurement; Measures; Metabolic; Metabolism; Monitor; Monkeys; Mus; Ophthalmology; Ophthalmoscopes; Ophthalmoscopy; Optical Coherence Tomography; Optics; Outcome; Pharmacological Treatment; Phototransduction; Pigments; Research; Resolution; Resources; Retina; Retinal; Retinal Diseases; Retinal Pigments; Retinoids; Risk; Safety; Scanning; Staging; Stem cells; Structure; Technology; Testing; Tissues; Treatment Effectiveness; Vertebrate Photoreceptors; Vision; Visual system structure; absorption; adaptive optics; adduct; base; cellular imaging; chromophore; combat; detector; drug discovery; fluorophore; follow-up; human subject; improved functioning; in vivo; in vivo imaging; instrument; light intensity; mouse model; new technology; novel; novel diagnostics; programs; prototype; relating to nervous system; research study; response; success; tissue regeneration; tool; two-photon; visual cycle

 DESCRIPTION (provided by applicant): One of the stated goals of the NEI Audacious Goal Initiative in addressing the technical needs and opportunities for imaging the visual system (U01) is "to develop new technology for the non-invasive measurement of retina function in vivo as related to natural vision, including visualization of metabolism at the cellular level and electrical activities". Based on strong newly acquired preliminary data, this application proposes the development of a new instrument that can image the retina in exceptional detail that together with two highly sensitive related tests can be used to evaluate retinal function. The specific aims are: (A) with a mouse two-photon ophthalmoscope (TPO), develop a novel diagnostic paradigm used to assess two functional aspects of vision: the metabolic flow of retinoids through the retinoid cycle and visual sensitivity. Along with mass spectrometric analyses, we will define critical intersections between images, structure, function and toxic molecules that affect the retina and identify potential therapies that preserve this neural tissue; and (B) develop and test a TPO imaging and diagnostic tool in humans. Initial tests will be done in a surrogate monkey/pig eye and then with the knowledge gained, we will image the eyes of individuals with advanced stages of blinding retinal diseases. Despite its' `audacity', this proposal can realistically be completed during the funding cycle. The scientific questions and interim milestones required to make this program successful are presented herein.

 描述（由应用程序提供）：Nei Audacious目标计划的既定目标之一是解决视觉系统成像的技术需求和机会（U01）是“开发新技术，用于在体内对视网膜功能的非侵入性测量与自然视觉相关，包括在细胞和电气活动中的代谢性可视化，包括对自然视觉的可视化。”基于强大的新获得的初步数据，该应用程序提出了一种新仪器的开发，该工具可以以特殊的细节对视网膜进行成像，并可以使用两个高度敏感的相关测试来评估残留功能。具体目的是：（a）用小鼠双光子眼镜（TPO）开发出一种新型的诊断范式，用于评估视觉的两个功能方面：类维质循环和视觉敏感性的代谢流动。除了质谱分析外，我们将定义影响视网膜的图像，结构，功能和有毒分子之间的关键交叉点，并鉴定保留这种神经组织的潜在疗法。 （b）开发和测试人类中的TPO成像和诊断工具。初始测试将在替代猴子/猪眼中进行，然后在获得知识的情况下，我们将对具有盲目残留疾病的高级阶段的眼睛进行想象。尽管它的“大胆”，但该提案仍可以在资金周期内实际完成。在这里提出了使该计划成功的科学问题和临时里程碑。