SBIR Phase II: Commercialization and Scale Up of Ultra Low Fouling and Functionalizable Surface Coatings Based on Zwitterionic Polycarboxybetaine

这个小型企业创新研究（SBIR）第二阶段项目旨在展示两性离子技术在蛋白质相互作用分析中的非凡性能。两性离子聚羧基甜菜碱（pCB）不仅具有很强的抗非特异性蛋白质吸附能力，而且具有丰富的功能基团，可以通过常规化学方法方便、有效地固定生物分子。这种双功能特性将pCB与其他现有的低污染材料区分开来，并使复杂介质中的诊断或分子识别成为可能。本项目将以低成本、简单的生产工艺，实现高质量的两性离子材料和涂料的规模化生产。预期的结果是，人们可以直接将分子识别元件固定在无污染的背景上，以实现广泛的应用。该项目的更广泛/商业影响将是为生物医学和工程应用提供一种有效的方法，以保护表面免受复杂介质中不必要的相互作用。两性离子技术具有成本低、稳定性好、有效性高和附加功能多等上级优点。对于蛋白质相互作用分析和医学诊断，该技术将提高生物传感器的灵敏度和特异性，并能够检测未稀释的人血浆和血清中的分析物。

This Small Business Innovation Research (SBIR) Phase II project aims to demonstrate the extraordinary performance of zwitterionic technology in protein interaction analysis. Zwitterionic polycarboxybetaine (pCB) is not only highly resistant to nonspecific protein adsorption, but also has abundant functional groups for the convenient and effective immobilization of biomolecules via conventional chemistry. This dual-functional property distinguishes pCB from other existing low-fouling materials, and enables diagnostics or molecular recognition in complex media. In this project, high-quality zwitterionic materials and coatings will be scaled up with low-cost and simple production process. The expected outcome is that one can immobilize molecular recognition elements directly onto a non-fouling background for a wide range of applications.The broader/commercial impacts of this project will be the potential to offer an effective approach in protection of surfaces from unwanted interactions in complex media for biomedical and engineering applications. Zwitterionic technology has superior advantages of low cost, stability, effectiveness and additional functionalities. For protein interaction analysis and medical diagnostics, this technology will improve the sensitivity and specificity of a biosensor, and enable the detection of analytes in undiluted human blood plasma and serum.