Exploring controlled drug release from magneto-liposomes by alternating and pulsed magnetic fields

通过交变和脉冲磁场探索磁脂质体的受控药物释放

• 批准号: 1128570
• 负责人: Viktor Chikan
• 金额: $ 33.38万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1128570&HistoricalAwards=false
• 关键词: Exploring; controlled; drug; release; magneto

Intellectual Merit: The aim of the proposal is to explore controlled disruption of the outer membranes of unilamellar lipsomes by using the mechanical rotation of superparamagnetic Fe/Fe3O4 nanorods, which will act as nano stir bars. The mechanical rotation of the nanoparticles will be driven by both continuous and pulsed magnetic fields. The hypothesis is that the unilamellar bilayers of the liposomes will be disrupted by the magnetic field induced motion of the nano stir bars, resulting in the quick release of the hydrophilic payload without a significant increase in temperature. This approach will permit the delivery of thermosensitive drugs (e.g. anticancer drugs) to their biological targets.The objective of this proposed research is to improve the basic understanding of how mechanical movement of magnetic nanoparticles can trigger biologically relevant processes in order to optimize magneto-liposomes for pharmaceutical delivery. Experimental plans to generate strong and pulsed magnetic fields are presented in this proposal. In situ dynamic light scattering of liposomes treated with continuous alternating magnetic fields will be used to monitor the size and phase stability of the liposomes. In situ Faraday rotation and time-resolved fluorescence experiments will help obtain dynamical information of the inner viscosities of core- and membrane-regions, as well as dissolution-reconstruction of supramolecular lipid bilayer/magnetic nanoparticle assemblies upon exposure to short pulsed magnetic fields. The proposed drug delivery by mechanically triggered liposomes will be able to deliver virtually any drug to any position of the body. Broader Impact: The results from the project could potentially lead to a new physical method to produce controlled release of drugs by magnetic fields. In addition, the project will provide quantitative data how the membrane fluidity is affected by the mechanical motion of magnetic nanoparticles. The Department of Chemistry at Kansas State University will create one block course for the duration of one week in order to enhance their students? perspective on biophysical chemistry and nanomedicine: The PI and Co-PI will develop and teach a block course entitled ?Nanoparticles as Payloads and Triggers for Release in Cancer Therapy? during the spring semester as a part of the ?Material Chemistry Course?. This course will be open to all graduate and undergraduate students in veterinary medicine, chemistry, biochemistry, biology and physics. The research will help extend this research to ongoing collaborative efforts on magnetic hyperthermia at Kansas State University. The research will also create opportunities to incoming REU (Research Experience of Undergraduates), SUROP (Summer Undergraduate Research Opportunity Program) and DSP (Developing Scholars? Program) students at Kansas State University. The proposed instrumental development of a pulsed magnetic field system will create a unique facility in the Midwestern United States, which will be open to collaborations with other universities and research institutions.

智力优势：该提案的目的是探索通过使用超顺磁性Fe/Fe 3 O 4纳米棒的机械旋转来控制单层脂质体外膜的破裂，该纳米棒将充当纳米搅拌棒。 纳米颗粒的机械旋转将由连续和脉冲磁场驱动。假设是脂质体的单层双层将被纳米搅拌棒的磁场诱导运动破坏，导致亲水性有效载荷的快速释放，而温度没有显著升高。这种方法将允许交付的热敏药物（如抗癌药物）的生物targets. Objective本拟议的研究是提高磁性纳米粒子的机械运动如何触发生物相关的过程，以优化磁性脂质体药物输送的基本理解。实验计划，以产生强和脉冲磁场中提出的建议。用连续交变磁场处理的脂质体的原位动态光散射将用于监测脂质体的大小和相稳定性。原位法拉第旋转和时间分辨荧光实验将有助于获得的核心和膜区域的内部粘度的动力学信息，以及超分子脂质双层/磁性纳米粒子组件暴露于短脉冲磁场后的溶解重建。所提出的通过机械触发脂质体的药物递送将能够将几乎任何药物递送到身体的任何位置。更广泛的影响：该项目的结果可能会导致一种新的物理方法，通过磁场来控制药物的释放。此外，该项目还将提供磁性纳米颗粒的机械运动如何影响膜流动性的定量数据。堪萨斯州立大学的化学系将开设一个为期一周的单元课程，以提高他们的学生？生物物理化学和纳米医学的角度：PI和合作PI将开发和教一个块课程题为？纳米粒子作为癌症治疗的有效载荷和释放触发器？作为春季学期的一部分材料化学课程？本课程将开放给兽医学，化学，生物化学，生物学和物理学的所有研究生和本科生。这项研究将有助于将这项研究扩展到堪萨斯州立大学正在进行的磁热疗合作。该研究还将创造机会，以传入REU（本科生的研究经验），SUROP（夏季本科生研究机会计划）和DSP（发展学者？计划）的学生在堪萨斯州立大学。拟议的脉冲磁场系统的仪器开发将在美国中西部建立一个独特的设施，该设施将与其他大学和研究机构合作。