Photosynthetic conversion of CO2 and low grade heat from biorefineries into linalool

生物精炼厂的二氧化碳和低品热通过光合作用转化为芳樟醇

• 批准号: 1133951
• 负责人: Ruanbao Zhou
• 金额: $ 30万
• 依托单位: South Dakota State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-11-01 至 2016-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1133951&HistoricalAwards=false
• 关键词: Photosynthetic; conversion; CO2; low; grade

PI: Ruanbao ZhouProposal Number: 1133951Intellectual MeritBiorefineries typically release about one third of the carbohydrate carbon as carbon dioxide (CO2) during fermentation. Ideally, a photosynthetic microorganism could be engineered to convert CO2 directly into liquid transportation fuels. Unfortunately, current approaches for using photosynthetic microorganisms to make fuel from CO2 suffer from many technical challenges, including the need to harvest cells, extract the energy-dense oils, and then convert the oil into the final product. To address these challenges, the proposed research will genetically engineer two cyanobacteria (Anabaena sp. PCC7120 and Nostoc punctiforme) to directly produce and secrete linalool, a ten-carbon terpene alcohol, through the non melavonate (MEP) pathway using sunlight as the energy source and CO2 as the carbon feedstock. To intensify linalool production, efforts will also be made to genetically engineer the cyanobacteria to increase the rate of photosynthetic CO2 fixation, and to re-direct the carbon flow to linalool synthesis by shutting unnecessary pathways that compete for fixed carbon. Towards this end, the proposed research plan has three components: 1) stable insertion of optimal LinS gene into chromosome of cyanobacteria; 2) correct metabolic flux to maximize linalool productivity; 3) assess linalool production under both photosynthetic andheterotrophic conditions. The performance of the engineered strains will be assessed in a recirculating photobioreactor system that uses phase separation for linalool recovery. Linalool yield and productivity will be compared to input requirements.Broader ImpactsThe proposed education and outreach activities will focus on providing opportunities for Native American students through the 2+2+2 Program at the South Dakota State University (SDSU). First, week-long activities in metabolic engineering will be developed as part of the summer institute program. Through these activities, Native American high school students and their teachers will be recruited to participate in 6-8 week long, summer research internships. Teachers will use the research experience to develop classroom/lab activities to share with their students. Students will participate in on-going projects, and then will be given the opportunity to present their efforts at scientific meetings organized through the South Dakota Academy of Science and SDSU?s Undergraduate Research Day. Native American undergraduate students who attend tribal colleges and then transfer to SDSU will also be offered summer research experiences.

PI：阮宝周建议编号：1133951智力价值生物精炼厂通常在发酵过程中将大约三分之一的碳水化合物碳释放为二氧化碳(CO2)。理想情况下，可以设计一种光合作用微生物，将二氧化碳直接转化为液体运输燃料。不幸的是，目前利用光合作用微生物从二氧化碳中提取燃料的方法面临着许多技术挑战，包括需要采集细胞，提取高能量密度的石油，然后将石油转化为最终产品。为了应对这些挑战，拟议的研究将对两种蓝藻(鱼腥藻)进行基因工程。PCC7120和点状念珠菌)，以阳光为能源，二氧化碳为碳源，通过MEP途径直接生产和分泌芳樟醇，这是一种十碳萜醇。为了加强芳樟醇的生产，还将努力对蓝藻进行基因工程，以提高光合作用二氧化碳的固定速率，并通过关闭竞争固定碳的不必要途径，将碳流重新引导到芳樟醇合成。为此，建议的研究计划包括三个部分：1)将优化的LINS基因稳定地插入蓝藻的染色体中；2)纠正代谢通量以使芳樟醇产量最大化；3)评估光合作用和异养条件下芳樟醇的产量。工程菌株的性能将在循环光生物反应器系统中进行评估，该系统使用相分离来回收芳樟醇。芳樟醇产量和生产率将与投入需求进行比较。布罗德影响拟议的教育和推广活动将侧重于通过南达科他州立大学(SDSU)的2+2+2计划为美洲原住民学生提供机会。首先，为期一周的代谢工程活动将作为暑期学院计划的一部分进行开发。通过这些活动，美国原住民高中生和他们的老师将被招募参加为期6-8周的暑期研究实习。教师将利用研究经验开展课堂/实验室活动，与学生分享。学生将参与正在进行的项目，然后将有机会在通过南达科他州科学院和南加州大学S本科生研究日组织的科学会议上展示他们的努力。在部落学院就读，然后转到南加州大学的美国原住民本科生也将获得暑期研究体验。