Analyses of the RNA polymerase I (Pol I) machinery: specific transcriptional mechanisms and comparison with other RNA polymerases (A01)

RNA 聚合酶 I (Pol I) 机制的分析：特定转录机制以及与其他 RNA 聚合酶的比较 (A01)

• 批准号: 202752861
• 金额: --
• 依托单位: Lehrstuhl für Biochemie III
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/202752861?language=en
• 关键词: Analyses; RNA; polymerase; Pol; machinery

RNA polymerase I (Pol I) is a specialized enzyme which transcribes the ribosomal RNA (rRNA) genes in all eukaryotes investigated so far. This project aims to unravel specific features of the Pol I machinery and to gain more insights into the mechanism of Pol I dependent transcription elongation and termination. Analyses of how structural and functional differences between the three eukaryotic nuclear RNA polymerases influence transcription of various nucleoprotein templates will be continued on a molecular level and will include Pol I mutants and templates of higher complexity.

RNA聚合酶I （RNA polymerase I, Pol I）是一种在所有真核生物中转录核糖体RNA （rRNA）基因的特殊酶。本项目旨在揭示Pol I机制的具体特征，并深入了解Pol I依赖的转录延伸和终止机制。三种真核生物核RNA聚合酶之间的结构和功能差异如何影响各种核蛋白模板的转录的分析将继续在分子水平上进行，并将包括Pol I突变体和更高复杂性的模板。