EAGER: Isolation of a C. Elegans Germ Cell Line

EAGER：线虫生殖细胞系的分离

• 批准号: 1138454
• 负责人: Edward Kipreos
• 金额: $ 30万
• 依托单位: University of Georgia Research Foundation Inc
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1138454&HistoricalAwards=false
• 关键词: EAGER; Isolation; C.; Elegans; Germ

Intellectual MeritModel organisms are widely used in biology research to understand how fundamental biological processes occur in all animals, including humans. The nematode C. elegans is one of the foremost model organisms, and has been used for groundbreaking insights in multiple areas of biology. C. elegans has many advantages as an experimental system, including powerful genetic approaches, but it lacks an essential tool: there are no C. elegans cell lines. Cell lines are cells that have been isolated from the animal and are able to continuously divide in a tissue culture system. C. elegans cell lines would allow for many experimental approaches that cannot be performed with the whole animal, including biochemical experiments with a homogeneous cell population, RNAi-based gene inactivation screens for cellular defects, and small molecule screens for bioactive compounds that modulate molecular pathways. The cell lines would be available for use by hundreds of laboratories to extend our knowledge of basic processes in a wide array of biological areas. This project seeks to isolate C. elegans cell lines from stem cells of the reproductive system. Broader ImpactsThe project will enhance the research infrastructure by providing a powerful new tool (C. elegans cell lines) that can be used to probe many areas of biological research. Additionally, local K-12 science teachers will participate in summer internships where they will contribute to the research to create a C. elegans cell line. In addition to broadening their research experience, the teachers will learn how to work with C. elegans, which can be used in elementary and high school laboratory courses to effectively teach genetics. Finally, college students will also be involved in carrying out the research, which will contribute to the training of the next generation of scientists.

智力优异模型生物广泛用于生物学研究，以了解包括人类在内的所有动物的基本生物过程。 线虫C.线虫是最重要的模式生物之一，并已被用于生物学多个领域的突破性见解。 C.作为一个实验系统，秀丽隐杆线虫有许多优势，包括强大的遗传方法，但它缺乏一个必要的工具：没有C。elegans细胞系。 细胞系是从动物中分离的细胞，能够在组织培养系统中连续分裂。 C. elegans细胞系将允许许多不能用整个动物进行的实验方法，包括用同质细胞群进行的生物化学实验，基于RNAi的细胞缺陷基因失活筛选，以及调节分子途径的生物活性化合物的小分子筛选。 这些细胞系将可供数百个实验室使用，以扩展我们在广泛的生物领域的基本过程的知识。 该项目旨在分离C。elegans细胞系从生殖系统的干细胞。 更广泛的影响该项目将通过提供强大的新工具（C。elegans细胞系），其可用于探测生物学研究的许多领域。 此外，当地的K-12科学教师将参加暑期实习，他们将为研究做出贡献，以创建一个C。elegans细胞系 除了扩大他们的研究经验，教师将学习如何与C。elegans，可用于小学和高中实验室课程，有效地教授遗传学。 最后，大学生也将参与开展研究，这将有助于培养下一代科学家。