cGMP signalling in Plasmodium intracellular development

疟原虫细胞内发育中的 cGMP 信号传导

• 批准号: 1146221
• 负责人: Purnima Bhanot
• 金额: $ 96万
• 依托单位: Rutgers, The State University of New Jersey-RBHS-New Jersey Med
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146221&HistoricalAwards=false
• 关键词: cGMP; signalling; Plasmodium; intracellular; development

Malaria kills nearly a million people a year, mostly young children. There is no vaccine against malaria and currently used anti-malarial drugs face several challenges. Malaria is caused by parasites called Plasmodium, which are transmitted to humans via mosquito bites. Inside the human body, Plasmodium's first act is to infect the liver. Inside the liver cells, each parasite divides to form tens of thousands of more parasites in just 2-3 weeks, which are eventually released into the blood. How does the parasite control this remarkable process? The investigator has previously discovered an enzyme that is essential for Plasmodium's development in the liver. Without it, parasites are "arrested" within the liver cell. In this project, the investigator will use molecular biology, genetics and advanced microscopy to reveal the exact function of the enzyme. The investigator will determine the genes that are turned on or off in response to the enzyme, and identify the proteins that partner with this enzyme to enable the parasite's development in the liver. This knowledge gained by this project will, in the future, help scientists design new ways to block the parasite's infection of the liver and treat malaria.Carrying out this work at the New Jersey Medical School will introduce underrepresented undergraduate, graduate and medical students from the Newark area to biomedical research. The project will include local undergraduate and high schools students recruited through summer internship programs, with a view towards developing a life-long interest in the scientific enterprise.

疟疾每年夺去近100万人的生命，其中大部分是儿童。没有针对疟疾的疫苗，目前使用的抗疟疾药物面临着一些挑战。疟疾是由称为疟原虫的寄生虫引起的，疟原虫通过蚊子叮咬传播给人类。在人体内，疟原虫的第一个行动是感染肝脏。在肝细胞内，每种寄生虫在短短2-3周内分裂形成数万种寄生虫，最终释放到血液中。 寄生虫是如何控制这个非凡的过程的呢？研究人员先前发现了一种对疟原虫在肝脏中发育至关重要的酶。没有它，寄生虫在肝细胞内被“逮捕”。在这个项目中，研究人员将使用分子生物学，遗传学和先进的显微镜来揭示酶的确切功能。研究人员将确定响应酶而打开或关闭的基因，并确定与这种酶合作的蛋白质，以使寄生虫在肝脏中发育。该项目获得的知识将在未来帮助科学家设计新的方法来阻止寄生虫感染肝脏和治疗疟疾。在新泽西医学院开展这项工作将介绍来自纽瓦克地区的代表性不足的本科生、研究生和医科学生进行生物医学研究。 该项目将包括通过暑期实习计划招募的当地本科生和高中生，以期培养对科学事业的终身兴趣。