Mechanisms of shoot apical function in gametophyte dominant and sporophyte dominant model plants

配子体优势和孢子体优势模型植物茎尖功能的机制

• 批准号: 1146733
• 负责人: Michael Scanlon
• 金额: $ 3.08万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1146733&HistoricalAwards=false
• 关键词: Mechanisms; shoot; apical; function; gametophyte

Unlike animals, all land plants have a haploid gametophyte stage of their life cycle. Gametophytes are multicellular plants that contain one half the chromosome complement of the diploid phase, the sporophyte. In bryophytes like mosses, the gametophyte is dominant, while in familiar angiosperms like maize the diploid sporophyte dominates. In all cases, development of above ground structures depends upon the activity of apical meristems, pools of self-propagating stem cells located at the growing plant tips. Widespread variation in meristem structure has evolved across these wide groups; despite this structural variation, meristem function during organogenesis and stem cell renewal is conserved. Major evolutionary innovations in shoot development include the dominance of the sporophyte generation over the gametophyte, and the emergence of multicellular shoot apical meristems that produce photosynthetic leaves. In this project, analyses of gene expression networks in plant shoot apices will identify shared and unique mechanisms for apical meristematic development among the ancient gametophyte-dominant model bryophytes Marchantia polymorpha and Physcomitrella patens, and the recently evolved sporophyte-dominant angiosperms Zea mays and Arabidopsis thaliana. Laser-microdissection coupled with RNA-sequencing will compare shoot apical transcriptomic signatures from these diverse model plants. Three categories of candidate gene will be identified: (1) functionally shared transcripts among the gametophyte meristems or lateral organs of the bryophytes and the sporophyte meristem or lateral organ of maize and Arabidopsis; (2) sporophyte-specific transcripts among the four model organisms, and; (3) transcripts unique to each lineage and domain. All data generated during this project will be released to public databases, including the TRANSCRIPTOME link at the CosMoss database for Physcomitrella patens, and the Marchantia polymorpha database at the DOE Joint Genome Institute. This project also will provide training in genomic analyses and mechanisms of plant development for a Cornell University Plant Biology graduate student.

与动物不同，所有的陆生植物在其生命周期中都有一个单倍体配子体阶段。配子体是多细胞植物，其含有二倍体阶段（孢子体）的一半染色体。 在苔藓类植物中，配子体占优势，而在玉米等常见被子植物中，二倍体孢子体占优势。 在所有情况下，地上结构的发育取决于顶端分生组织的活性，顶端分生组织是位于生长中的植物顶端的自繁殖干细胞池。 分生组织结构的广泛变异已经在这些广泛的群体中进化;尽管这种结构变异，但分生组织在器官发生和干细胞更新过程中的功能是保守的。 芽发育的主要进化创新包括孢子体世代对配子体的优势，以及产生光合叶的多细胞芽顶端分生组织的出现。在这个项目中，基因表达网络在植物茎尖的分析将确定共享和独特的机制，顶端分生组织的发展之间的古老的配子体占主导地位的模式，地钱和小立碗藓，和最近进化的孢子体占主导地位的被子植物玉米和拟南芥。 激光显微切割结合RNA测序将比较这些不同模式植物的茎尖转录组特征。 将鉴定三类候选基因：（1）在苞片的配子体分生组织或侧生器官以及玉米和拟南芥的孢子体分生组织或侧生器官中功能共享的转录物;（2）在四种模式生物中孢子体特异性转录物，以及;（3）每个谱系和结构域独特的转录物。 该项目期间生成的所有数据将发布到公共数据库，包括CosMoss数据库中的小立碗藓（Physcomitrella patens）的TRANSPORTOME链接，以及美国能源部联合基因组研究所的多形地钱（Marchantia polymorpha）数据库。该项目还将为康奈尔大学植物生物学研究生提供基因组分析和植物发育机制方面的培训。