Genomic Analyses of Shoot Meristem Function in Maize

主要研究者：迈克尔·斯坎隆（康奈尔大学）CoPI：玛丽亚·蒂默曼斯（冷泉港实验室），加里米尔鲍尔（明尼苏达大学），Jianming Yu（堪萨斯州立大学），黛安·贾尼克-巴克纳，乔恩·贝克（杜鲁门州立大学）合作者丹内特尔顿（爱荷华州州立大学），多琳·韦尔（USDA-ARS/冷泉港实验室），大卫米克洛斯（多兰DNA学习中心）茎顶端分生组织（SAM）负责植物所有地上器官的发育。尽管其在器官发生过程中的重要作用，但调节SAM功能的分子机制知之甚少。由于生物学优势和基因组资源的独特组合，玉米特别适合于在基因组尺度上研究SAM结构、功能和异质性的遗传网络。 本项目将致力于以下五个方面的研究：（1）SAM基因功能调控和玉米叶型形成的候选基因的遗传特征;（2）利用激光显微切割和转录谱分析技术鉴定SAM启动和发育过程中表达的基因;（3）SAM分生组织功能中小RNA控制网络的鉴定;（4）SAM分生组织功能中小RNA控制网络的鉴定;（5）SAM分生组织功能中小RNA控制网络的鉴定。（4）控制SAM结构的数量性状基因座（QTL）的鉴定;和（5）新等位基因的鉴定（单倍型）有助于复杂的叶性状使用连锁不平衡作图。SAM功能的这些基因组分析将为旨在传播对植物科学的理解和欣赏的科学培训和公共宣传战略提供知识框架。在杜鲁门州立大学，一个小的，公共文科院校在柯克斯维尔（MO）的本科生将接受培训，以执行新的SAM基因的生物信息学注释，并进行遗传，表型和分子分析的玉米SAM突变体在此项目期间产生的。冷泉港实验室的多兰DNA学习中心将建立一个名为“杂草奇迹”的公共网站/播客，介绍玉米遗传研究的历史，并庆祝其对人类文化的影响。 该项目期间产生的所有数据将发布到公共数据库，包括MaizeGDB（www.maizegdb.org）和Gramene（www.gramene.org），携带新发现的玉米突变的种子将公开发布。

PI: Michael Scanlon (Cornell University)CoPIs: Marja Timmermans (Cold Spring Harbor Laboratory), Gary Muehlbauer (University of Minnesota), Jianming Yu (Kansas State University), Diane Janick-Buckner, Jon Beck (Truman State University)Collaborators Dan Nettleton (Iowa State University), Doreen Ware (USDA-ARS/Cold Spring Harbor Laboratory), David Micklos (Dolan DNA Learning Center)The shoot apical meristem (SAM) is responsible for development of all above ground organs in the plant. Despite its essential roles during organogenesis, the molecular mechanisms regulating SAM function are poorly understood. Owing to a unique combination of biological advantages and genomic resources, maize is particularly well positioned for investigations of the genetic networks underlying SAM structure, function, and heterogeneity on a genomic scale. This project will address five specific objectives: (1) genetic characterization of candidate genes implicated in the regulation of SAM gene function and maize leaf patterning; (2) use of laser microdissection and transcript profiling to identify genes expressed during SAM initiation and development ; (3) identification of small RNA-controlled networks in SAM meristem function; (4) identification of quantitative trait loci (QTLs) controlling SAM architecture; and (5) identification of novel alleles (haplotypes) contributing to complex leaf traits using linkage-disequilibrium mapping.Broader impacts of proposed research: These genomic analyses of SAM function will provide the intellectual framework for a strategy of scientific training and public outreach designed to disseminate an understanding and appreciation of plant science. Undergraduates at Truman State University, a small, public liberal arts institution in Kirksville (MO) will be trained to perform bioinformatic annotations of novel SAM genes, and carry out genetic, phenotypic, and molecular analyses of maize SAM mutants generated during this project. The Dolan DNA Learning Center at Cold Spring Harbor Laboratory will construct a public website/podcast entitled "Weed to Wonder" that will present the history of maize genetic research and celebrate its impact on human culture. All data generated during this project will be released to public databases include MaizeGDB (www.maizegdb.org) and Gramene (www.gramene.org), and seed harboring newly discovered maize mutations will be released for public distribution.