PECASE: Dielectric Phenotyping of Bacteria for Energy and Medicine

PECASE：用于能源和医学的细菌介电表型分析

• 批准号: 1150615
• 负责人: Cullen Buie
• 金额: $ 40万
• 依托单位: Massachusetts Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1150615&HistoricalAwards=false
• 关键词: PECASE; Dielectric; Phenotyping; Bacteria; Energy

Abstract1150615-Buie, Cullen R.The objective of the proposed research is to illuminate the coupling between cell envelope phenotype and dielectric properties. This will be achieved by the investigation of pathogenic bacteria and electrochemically active bacteria. A three-dimensional insulator based dielectrophoresis (3DiDEP) device will be used. The novel 3DiDEP platform enables non-invasive sorting, trapping, and characterization of living cells based upon the cell envelope phenotype. The hypothesis of the work is that 3DiDEP can be used to characterize the functions expressed on the bacteria cell envelope. 3DiDEP enables low voltage, high sensitivity separation and analysis of cells. Also, the proposed 3DiDEP employs electrodes outside of the microchannel, the technique operates at low frequency or DC electric fields. The proposed 3DiDEP is a powerful technique for high throughput phenotyping in microbiology. Phenotype based selection using 3DiDEP represents one of the first applications of microfluidics in the growing field of phenomics. The proposed project may also help to address widespread concerns in diverse fields ranging from renewable energy to healthcare. The PI also proposes to develop relationships with target universitis to increase the representation of underrepresented minorities in MIT graduate programs. The PI has outlined a unique approach to train a new generation of underrepresented minority scientists who may return to their respective undergraduate institutions as faculty, thereby attacking the diversity issue on both the student and faculty levels.

摘要1150615-Buie，Cullen R.拟议研究的目的是阐明细胞包膜表型和介电特性之间的耦合。 这将通过对病原菌和电化学活性细菌的研究来实现。 将使用基于三维绝缘体的介电泳（3DiDEP）装置。 新型3DiDEP平台能够基于细胞包膜表型对活细胞进行非侵入性分选、捕获和表征。 该工作的假设是，3DiDEP可用于表征细菌细胞包膜上表达的功能。 3DiDEP可实现低电压、高灵敏度的细胞分离和分析。 此外，所提出的3DiDEP采用微通道外部的电极，该技术在低频或DC电场下操作。 所提出的3DiDEP是用于微生物学中的高通量表型分型的强大技术。 使用3DiDEP的基于表型的选择代表了微流体在表型组学的不断发展的领域中的第一个应用。 拟议的项目还可能有助于解决从可再生能源到医疗保健等不同领域的广泛关注。 PI还建议与目标大学发展关系，以增加麻省理工学院研究生课程中代表性不足的少数民族的代表性。 PI概述了一种独特的方法来培养新一代代表性不足的少数民族科学家，他们可能会回到各自的本科院校担任教师，从而在学生和教师层面上解决多样性问题。