PFI:AIR - TT: A Platform for High Throughput Genetic Transformation of Bacteria
PFI:AIR - TT:细菌高通量遗传转化平台
基本信息
- 批准号:1640678
- 负责人:
- 金额:$ 20万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-09-15 至 2018-02-28
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This PFI: AIR Technology Translation project focuses on translating next generation microfluidic genetic transformation technologies to fill the need for high throughput generation of genetically modified microorganisms. This system will allow metabolic engineers to more rapidly develop microorganisms for the production of various bioengineered chemicals and materials. This novel genetic transformation system is important because the fields of synthetic biology and genetic engineering are currently limited by the ability to re-program microorganisms with foreign DNA. This project will result in a prototype high throughput genetic transformation platform to demonstrate the utility of the system. This genetic transformation platform will be able to process microorganisms nearly one hundred times faster than the current state of the art. This project addresses the following technology gap(s) as it translates from research discovery toward commercial application. Electroporation, cell permeabilization using pulsed electric fields, is an efficient way to deliver DNA constructs into microorganisms for genetic engineering and synthetic biology applications. Standard electroporation protocols involve parallel plate cuvettes to expose cell and DNA samples to uniform electric fields. However, testing different electroporation conditions involves hundreds of experiments (e.g. varying DNA construct, cell type, buffer composition), which is slow, labor-intensive, and expensive. This project aims to develop a prototype high throughput platform for genetic transformation of bacteria using microfluidic flow-through electroporation. The prototype device will be operated in 1) large scale production of bioengineered chemicals and 2) in a discovery mode to identify optimal transformation protocols for genetic engineering. This scalable technology will accelerate the development of new bioengineered chemicals that can be manufactured in a renewable manner. Personnel involved in this project, research scientists, postdocs, and undergraduate students, will receive innovation, entrepreneurship, and technology translation experiences through participation in prototype design, customer interviews, business plan competitions, engaging with mentors, and establishing a company to commercialize the technology. The project engages the MIT Venture Mentoring Services and the MIT Innovation Initiative to guide commercialization aspects in this technology translation effort from research discovery toward commercial reality.
该PFI:AIR技术翻译项目的重点是翻译下一代微流体遗传转化技术,以满足高通量生产转基因微生物的需求。该系统将允许代谢工程师更快地开发用于生产各种生物工程化学品和材料的微生物。 这种新的遗传转化系统很重要,因为合成生物学和基因工程领域目前受到用外源DNA重新编程微生物的能力的限制。该项目将产生一个原型高通量遗传转化平台,以展示该系统的实用性。这个基因转化平台将能够处理微生物的速度比现有技术快近100倍。该项目解决了以下技术差距,因为它从研究发现转化为商业应用。电穿孔,使用脉冲电场的细胞透化,是将DNA构建体递送到微生物中用于基因工程和合成生物学应用的有效方式。标准电穿孔方案涉及平行板比色皿以将细胞和DNA样品暴露于均匀电场。然而,测试不同的电穿孔条件涉及数百个实验(例如,不同的DNA构建体、细胞类型、缓冲液组成),这是缓慢的、劳动密集型的并且昂贵的。本项目旨在开发一个原型的高通量平台,用于使用微流体流通电穿孔进行细菌的遗传转化。该原型装置将用于1)生物工程化学品的大规模生产和2)以发现模式运行,以确定遗传工程的最佳转化方案。这种可扩展的技术将加速新的生物工程化学品的开发,这些化学品可以以可再生的方式生产。参与该项目的人员,研究科学家,博士后和本科生,将通过参与原型设计,客户访谈,商业计划竞赛,与导师合作以及建立公司将技术商业化来获得创新,创业和技术翻译经验。该项目与麻省理工学院创业指导服务和麻省理工学院创新计划合作,以指导从研究发现到商业现实的技术转化工作的商业化方面。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Cullen Buie其他文献
Randomly barcoded transposon mutant libraries for gut commensals II: Applying libraries for functional genetics
肠道共生菌的随机条形码转座子突变体文库II:将文库应用于功能遗传学
- DOI:
10.1016/j.celrep.2023.113519 - 发表时间:
2024-01-23 - 期刊:
- 影响因子:6.900
- 作者:
Carlos Geert Pieter Voogdt;Surya Tripathi;Stefan Oliver Bassler;Saria A. McKeithen-Mead;Emma R. Guiberson;Alexandra Koumoutsi;Afonso Martins Bravo;Cullen Buie;Michael Zimmermann;Justin L. Sonnenburg;Athanasios Typas;Adam M. Deutschbauer;Anthony L. Shiver;Kerwyn Casey Huang - 通讯作者:
Kerwyn Casey Huang
Randomly barcoded transposon mutant libraries for gut commensals I: Strategies for efficient library construction
肠道共生菌随机条形码转座子突变体文库I:高效文库构建策略
- DOI:
10.1016/j.celrep.2023.113517 - 发表时间:
2024-01-23 - 期刊:
- 影响因子:6.900
- 作者:
Surya Tripathi;Carlos Geert Pieter Voogdt;Stefan Oliver Bassler;Mary Anderson;Po-Hsun Huang;Nazgul Sakenova;Tümay Capraz;Sunit Jain;Alexandra Koumoutsi;Afonso Martins Bravo;Valentine Trotter;Michael Zimmerman;Justin L. Sonnenburg;Cullen Buie;Athanasios Typas;Adam M. Deutschbauer;Anthony L. Shiver;Kerwyn Casey Huang - 通讯作者:
Kerwyn Casey Huang
Cullen Buie的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Cullen Buie', 18)}}的其他基金
2019 Physics and Chemistry of Microfluidics Gordon Research Conference: Microfluidics for Studying Complex Systems
2019年微流控物理与化学戈登研究会议:用于研究复杂系统的微流控
- 批准号:
1929846 - 财政年份:2019
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
2016 Dielectrophoresis Meeting at MIT
2016年麻省理工学院介电泳会议
- 批准号:
1645534 - 财政年份:2016
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
I-Corps: Kytopen: Expanding the language of biology with pulsed electric fields
I-Corps:Kytopen:用脉冲电场扩展生物学语言
- 批准号:
1562925 - 财政年份:2015
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
2014 AES Electrophoresis Society Annual Meeting
2014年AES电泳学会年会
- 批准号:
1505644 - 财政年份:2015
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PECASE: Dielectric Phenotyping of Bacteria for Energy and Medicine
PECASE:用于能源和医学的细菌介电表型分析
- 批准号:
1150615 - 财政年份:2012
- 资助金额:
$ 20万 - 项目类别:
Continuing Grant
EAGER: Culturing the Uncultured: Custom Microfluidic Systems for Growth and Isolation of Environmental Microbes
EAGER:培养未培养的:用于环境微生物生长和分离的定制微流体系统
- 批准号:
1048133 - 财政年份:2010
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
相似国自然基金
湍流和化学交互作用对H2-Air-H2O微混燃烧中NO生成的影响研究
- 批准号:51976048
- 批准年份:2019
- 资助金额:61.0 万元
- 项目类别:面上项目
相似海外基金
PFI-TT: Demonstration of a Liquid Piston Gas Compressor/Expander for Compressed Air Energy Storage and CO2 Sequestration
PFI-TT:用于压缩空气储能和二氧化碳封存的液体活塞气体压缩机/膨胀器的演示
- 批准号:
1827517 - 财政年份:2018
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR-TT: Ultrapure Lignins Recovered from Paper-Mill Black Liquors as Renewable Biopolymers
PFI:AIR-TT:从造纸厂黑液中回收超纯木质素作为可再生生物聚合物
- 批准号:
1701128 - 财政年份:2017
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR-TT: Preflex versus Reflex Control of a Multijoint Robotic Exoskeleton
PFI:AIR-TT:多关节机器人外骨骼的预反射与反射控制
- 批准号:
1701230 - 财政年份:2017
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR-TT: Commercializing a new genre of Intelligent Science Stations for informal and formal learning
PFI:AIR-TT:将新型智能科学站商业化,用于非正式和正式学习
- 批准号:
1701107 - 财政年份:2017
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR-TT: PharmaFlux: Drug Evaluation on a Biomimetic Microfluidic Device
PFI:AIR-TT:PharmaFlux:仿生微流体装置的药物评估
- 批准号:
1701136 - 财政年份:2017
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR-TT: Microwave flow cytometer: monitor yeast cell growth and microbial contamination in fermentation
PFI:AIR-TT:微波流式细胞仪:监测发酵过程中的酵母细胞生长和微生物污染
- 批准号:
1640578 - 财政年份:2016
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR - TT: Vision-based ergonomic risk assessment of working postures
PFI:AIR - TT:基于视觉的工作姿势人体工程学风险评估
- 批准号:
1640633 - 财政年份:2016
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR-TT: Scale-up Synthesis of Nanoparticles for Manufacturing Microelectrodes and Sensing Films of Indoor Air Quality Chemiresistor Sensors
PFI:AIR-TT:用于制造室内空气质量化学电阻传感器的微电极和传感薄膜的纳米颗粒的放大合成
- 批准号:
1640669 - 财政年份:2016
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI: AIR - TT: Developing A Bio-based Rejuvenator to Revitalize Asphalt in Scrap Roofing Shingles
PFI:AIR - TT:开发生物基再生剂以再生废屋顶瓦中的沥青
- 批准号:
1640517 - 财政年份:2016
- 资助金额:
$ 20万 - 项目类别:
Standard Grant
PFI-AIR-TT: Wearable sleepwear for quantitative prognostication and noninvasive therapy of obstructive sleep apnea
PFI-AIR-TT:可穿戴睡衣,用于阻塞性睡眠呼吸暂停的定量预测和无创治疗
- 批准号:
1543226 - 财政年份:2015
- 资助金额:
$ 20万 - 项目类别:
Standard Grant