Small angle x-ray scattering studies of biological macromolecules at cryogenic temperatures
低温下生物大分子的小角X射线散射研究
基本信息
- 批准号:1152348
- 负责人:
- 金额:$ 52.77万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Continuing Grant
- 财政年份:2012
- 资助国家:美国
- 起止时间:2012-08-15 至 2017-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
A protein's biological job is related to its shape, thus insight can be gained into a protein's function by measuring its structure and how this structure changes as the protein interacts with other molecules. This task is challenging because many proteins are small, with diameters about ten thousand times less than the width of a human hair. The field of structural biology relies on exploiting experimental methods that provide accurate structural information on such small length scales. In the last decade, thanks to the availability of high intensity x-ray beams at national facilities (synchrotron sources) as well as the development of sophisticated analysis programs that return structural envelopes, small angle x-ray scattering (SAXS) has become a reliable tool for rapidly obtaining structures of proteins and other large biological molecules. The greatest strength of SAXS is its ability to report structures of biological macromolecules insolution, without the need for specialized sample preparation. A protein's shape can now be determined in a simple and straightforward way. However, the radiation used to measure the sample can also damage it, changing its structure. This problem has been encountered in other radiation-based methods, including x-ray crystallography and electron microscopy. For these latter fields, the use of cryo-freezing has reduced damage to tolerable levels: biological samples are protected from radiation at temperatures of ~100 K. The goal of this project is to design and construct two instruments that will prepare flash-frozen samples optimized for SAXS experiments: cryo-SAXS. Because frozen samples can tolerate longer exposure to radiation, without concern for radiation-induced structural changes, this method will allow structures to be obtained from even small amounts of precious sample. The successful demonstration of cryo-SAXS will garner tremendous interest within the large structural biology community. All plans/designs generated as a result of this project will be made public, through journal publication, presentations at conferences, posting on existing public forums for crystallography and SAXS, and webinars. Dissemination through use at synchrotron sources is also extremely effective, as a large number of users pass through and are exposed to new technologies through users' meetings, facility websites and workshops. A long-term goal of this project is to develop a kit that makes this technology easily available to the structural biology community.
蛋白质的生物学功能与其形状有关,因此,通过测量蛋白质的结构以及蛋白质与其他分子相互作用时这种结构如何变化,可以深入了解蛋白质的功能。这项任务具有挑战性,因为许多蛋白质很小,直径比人类头发的宽度还小一万倍。结构生物学领域依赖于开发实验方法,在如此小的长度尺度上提供准确的结构信息。在过去的十年中,由于高强度x射线束在国家设施(同步加速器源)的可用性以及返回结构包膜的复杂分析程序的发展,小角度x射线散射(SAXS)已成为快速获得蛋白质和其他大型生物分子结构的可靠工具。SAXS的最大优点是它能够报告生物大分子的结构,而不需要专门的样品制备。蛋白质的形状现在可以用一种简单直接的方法来确定。然而,用于测量样品的辐射也会破坏它,改变它的结构。这个问题在其他基于辐射的方法中也遇到过,包括x射线晶体学和电子显微镜。对于后一种领域,冷冻冷冻的使用将损害降低到可容忍的水平:生物样品在~100 K的温度下免受辐射。本项目的目标是设计和构建两种仪器,用于制备针对SAXS实验优化的速冻样品:cryo-SAXS。由于冷冻样品可以承受更长时间的辐射,而不用担心辐射引起的结构变化,因此这种方法可以从少量的珍贵样品中获得结构。cryo-SAXS的成功演示将引起结构生物学界的极大兴趣。该项目产生的所有计划/设计都将通过期刊出版、会议演讲、在现有的晶体学和SAXS公共论坛上发布以及网络研讨会等方式公开。通过同步加速器源的使用进行传播也极为有效,因为大量用户通过用户会议、设施网站和讲习班接触到新技术。该项目的长期目标是开发一套工具,使这项技术易于结构生物学社区使用。
项目成果
期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Quantifying radiation damage in biomolecular small-angle X-ray scattering
- DOI:10.1107/s1600576716005136
- 发表时间:2016-06-01
- 期刊:
- 影响因子:6.1
- 作者:Hopkins, Jesse B.;Thorne, Robert E.
- 通讯作者:Thorne, Robert E.
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
Lois Pollack其他文献
Virus Dynamics Studied by Time-Resolved Small Angle X-Ray Scattering
- DOI:
10.1016/j.bpj.2018.11.2321 - 发表时间:
2019-02-15 - 期刊:
- 影响因子:
- 作者:
Josue San Emeterio;Lois Pollack - 通讯作者:
Lois Pollack
Characterization of DNA and RNA Ion Atmospheres Using Multiple-Energy Asaxs
- DOI:
10.1016/j.bpj.2010.12.626 - 发表时间:
2011-02-02 - 期刊:
- 影响因子:
- 作者:
Suzette A. Pabit;Steve P. Meisburger;Li Li;Joshua M. Blose;Christopher D. Jones;Lois Pollack - 通讯作者:
Lois Pollack
Local DNA Sequence Controls the Asymmetry of DNA Unwrapping from Nucleosome Core Particles
- DOI:
10.1016/j.bpj.2017.11.3085 - 发表时间:
2018-02-02 - 期刊:
- 影响因子:
- 作者:
Alexander Mauney;Lois Pollack - 通讯作者:
Lois Pollack
Dimer formation in the blue light sensing protein Vivid
- DOI:
10.1016/j.bpj.2008.12.2705 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Jessica S. Lamb;Brian D. Zoltowski;Suzette A. Palin;Li Li;Brian R. Crane;Lois Pollack - 通讯作者:
Lois Pollack
Measuring the Dimensions of a Compact Kinetic Intermediate in the Folding Pathway of the GlmS Ribozyme
- DOI:
10.1016/j.bpj.2010.12.1510 - 发表时间:
2011-02-02 - 期刊:
- 影响因子:
- 作者:
Steve Meisburger;Krista Brooks;Suzette Pabit;Li Li;Joshua Blose;Ken Hampel;Lois Pollack - 通讯作者:
Lois Pollack
Lois Pollack的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('Lois Pollack', 18)}}的其他基金
IIBR RoL: Applying innovative structural tools to highlight RNA's structural dynamics as RNA-protein complexes self-assemble
IIBR RoL:应用创新的结构工具来突出 RNA-蛋白质复合物自组装时的结构动力学
- 批准号:
1930046 - 财政年份:2019
- 资助金额:
$ 52.77万 - 项目类别:
Standard Grant
Collaborative Research: Ultra-fast and multiplexed time-resolved hydroxyl radical footprinting of nucleic acids and proteins
合作研究:核酸和蛋白质的超快速、多重时间分辨羟基自由基足迹
- 批准号:
0852813 - 财政年份:2009
- 资助金额:
$ 52.77万 - 项目类别:
Standard Grant
CAREER: The Interaction of Ions with DNA: An X-ray Scattering Study
职业:离子与 DNA 的相互作用:X 射线散射研究
- 批准号:
0347220 - 财政年份:2004
- 资助金额:
$ 52.77万 - 项目类别:
Continuing Grant
ROW: Ultra-low Temperature Studies of Quadrupolar Systems
ROW:四极系统的超低温研究
- 批准号:
9109524 - 财政年份:1991
- 资助金额:
$ 52.77万 - 项目类别:
Standard Grant
相似国自然基金
原发性开角型青光眼中SIPA1L1促进小梁网细胞外基质蛋白累积升高眼压的作用机制
- 批准号:82371054
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
基于位场广义梯度张量的欧拉反褶积方法研究
- 批准号:41504098
- 批准年份:2015
- 资助金额:21.0 万元
- 项目类别:青年科学基金项目
关于Moment-Angle流形的几个问题
- 批准号:11401118
- 批准年份:2014
- 资助金额:23.0 万元
- 项目类别:青年科学基金项目
稳定同伦中的无限降阶法与moment-angle流形
- 批准号:11471167
- 批准年份:2014
- 资助金额:65.0 万元
- 项目类别:面上项目
双星中性原子探测图像在地磁暴期间的时序演化过程反演分析
- 批准号:40974100
- 批准年份:2009
- 资助金额:45.0 万元
- 项目类别:面上项目
相似海外基金
Morphological evolution of the phase separation induced by conversion and re-conversion reactions investigated by small-angle X-ray scattering
小角 X 射线散射研究转化和再转化反应引起的相分离的形态演化
- 批准号:
22K05300 - 财政年份:2022
- 资助金额:
$ 52.77万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Long non-coding RNA in action: watching Xist interact with its protein binding partners using time-resolved small-angle X-ray scattering
长非编码 RNA 的作用:使用时间分辨小角 X 射线散射观察 Xist 与其蛋白质结合伙伴相互作用
- 批准号:
557355-2021 - 财政年份:2022
- 资助金额:
$ 52.77万 - 项目类别:
Postgraduate Scholarships - Doctoral
Ultra Small-Angle X-ray Diffractometer
超小角度X射线衍射仪
- 批准号:
504929269 - 财政年份:2022
- 资助金额:
$ 52.77万 - 项目类别:
Major Research Instrumentation
Small-Angle X-ray Scattering
小角X射线散射
- 批准号:
505274593 - 财政年份:2022
- 资助金额:
$ 52.77万 - 项目类别:
Major Research Instrumentation
MRI: Acquisition of a High-Pressure, High-Temperature Small Angle X-Ray Scattering Instrument for Oklahoma
MRI:为俄克拉荷马州采购高压高温小角度 X 射线散射仪
- 批准号:
2216074 - 财政年份:2022
- 资助金额:
$ 52.77万 - 项目类别:
Standard Grant
MRI: Acquisition of Small/Wide-Angle and Non-Ambient X-Ray Diffraction Packages for Research and Education in Energy and Environmental Sustainability
MRI:获取小/广角和非环境 X 射线衍射包,用于能源和环境可持续发展的研究和教育
- 批准号:
2216240 - 财政年份:2022
- 资助金额:
$ 52.77万 - 项目类别:
Standard Grant
MRI: Acquisition of a Xeuss 3.0 with Genix 3D Small-Angle X-ray Scattering Instrument
MRI:使用 Genix 3D 小角度 X 射线散射仪采集 Xeuss 3.0
- 批准号:
2117657 - 财政年份:2021
- 资助金额:
$ 52.77万 - 项目类别:
Standard Grant
MRI: Acquisition of a High-Throughput Small Angle X-ray Scattering Instrument for Data-Driven Materials Design
MRI:获取用于数据驱动材料设计的高通量小角度 X 射线散射仪器
- 批准号:
2116265 - 财政年份:2021
- 资助金额:
$ 52.77万 - 项目类别:
Standard Grant
Small Angle X-ray Scattering (SAXS)
小角 X 射线散射 (SAXS)
- 批准号:
455115343 - 财政年份:2021
- 资助金额:
$ 52.77万 - 项目类别:
Major Research Instrumentation
Long non-coding RNA in action: watching Xist interact with its protein binding partners using time-resolved small-angle X-ray scattering
长非编码 RNA 的作用:使用时间分辨小角 X 射线散射观察 Xist 与其蛋白质结合伙伴相互作用
- 批准号:
557355-2021 - 财政年份:2021
- 资助金额:
$ 52.77万 - 项目类别:
Postgraduate Scholarships - Doctoral