Novel Pt(II) Reagents for RNA Biochemistry

有了这个奖项，生命过程计划的化学资助俄勒冈州大学的维多利亚DeRose教授与Michael Haley教授合作研究，开发和使用Pt（II）试剂进行RNA交联，以帮助确定RNA的三级结构。 研究人员将采用过渡金属络合物合成，RNA加合研究和更全球化的化学定向RNA标记的组合。 还设想了后内收、第二阶段标记的要素。 化学，分子生物学和光谱技术的组合将被用来梳理出三级结构信息从通过这种化学生物学方法产生的标记RNA。如果成功的话，这种方法可能会发展成为核酸结构生物学领域更广泛适用的化学生物学工具，这是一种潜在的重要科学影响。 该项目还提供了学生共同指导的机会，在合成无机/有机化学家和RNA化学生物学家的指导下，在金属-核酸相互作用和光谱学方面有着良好的记录。 更广泛的教育影响还包括一个值得注意的本科生研究部分，并继续努力使妇女和传统上代表性不足的群体成员接触现代研究科学。

With this award, the Chemistry of Life Processes Program is funding Professor Victoria DeRose of the University of Oregon for collaborative studies with Professor Michael Haley to develop and use Pt(II) reagents for RNA-crosslinking to aid in the determination of RNA tertiary structure. The researchers will employ a combination of transition metal complex synthesis, RNA adduction studies, and more global, chemically directed RNA-tagging. Elements of post-adduction, second-stage tagging are also envisioned. A combination of chemical, molecular biological and spectroscopic techniques will be used to tease out tertiary structural information from the tagged RNAs generated through this chemical biology approach. If successful, this approach may grow into a more broadly applicable chemical biology tool for the field of nucleic acid structural biology, a potentially important scientific broader impact. The project also offers the opportunity for co-mentorship of students, under both a synthetic inorganic/organic chemist and an RNA chemical biologist with a strong record in metal-nucleic acid interactions and spectroscopy. The educational broader impacts also comprise a notable undergraduate research component and continued efforts to expose women and members of traditionally underrepresented groups to modern research science.