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Novel Pt(II) Reagents for RNA Biochemistry

用于 RNA 生物化学的新型 Pt(II) 试剂

基本信息

批准号：
1153147
负责人：
Victoria DeRose
金额：
$ 42万
依托单位：
University of Oregon Eugene
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2012
资助国家：
美国
起止时间：
2012-02-01 至 2015-01-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1153147&HistoricalAwards=false
关键词：
Novel Pt II Reagents RNA

项目摘要

With this award, the Chemistry of Life Processes Program is funding Professor Victoria DeRose of the University of Oregon for collaborative studies with Professor Michael Haley to develop and use Pt(II) reagents for RNA-crosslinking to aid in the determination of RNA tertiary structure. The researchers will employ a combination of transition metal complex synthesis, RNA adduction studies, and more global, chemically directed RNA-tagging. Elements of post-adduction, second-stage tagging are also envisioned. A combination of chemical, molecular biological and spectroscopic techniques will be used to tease out tertiary structural information from the tagged RNAs generated through this chemical biology approach. If successful, this approach may grow into a more broadly applicable chemical biology tool for the field of nucleic acid structural biology, a potentially important scientific broader impact. The project also offers the opportunity for co-mentorship of students, under both a synthetic inorganic/organic chemist and an RNA chemical biologist with a strong record in metal-nucleic acid interactions and spectroscopy. The educational broader impacts also comprise a notable undergraduate research component and continued efforts to expose women and members of traditionally underrepresented groups to modern research science.

通过这一奖项，生命过程化学计划资助俄勒冈大学的维多利亚·德罗斯教授与迈克尔·黑利教授合作研究，开发和使用用于RNA交联的铂(II)试剂，以帮助确定RNA的三级结构。研究人员将采用过渡金属络合物合成、RNA加合研究和更全局的、化学指导的RNA标记相结合的方法。还设想了后引证、第二阶段标记的要素。化学、分子生物学和光谱技术的组合将被用来从通过这种化学生物学方法产生的标记RNA中梳理出三级结构信息。如果成功，这种方法可能会成长为一种更广泛适用于核酸结构生物学领域的化学生物学工具，可能会产生重要的科学影响。该项目还为学生提供共同指导的机会，在一名合成无机/有机化学家和一名在金属-核酸相互作用和光谱学方面有良好记录的RNA化学生物学家的指导下。更广泛的教育影响还包括显著的本科生研究部分，以及继续努力让妇女和传统上代表性不足的群体的成员接触现代研究科学。