CAREER: Metal-Binding Sites in Nucleic Acids and Polymerases

这个职业奖在无机、生物无机和有机金属化学项目中支持德克萨斯农工大学维多利亚·j·德罗斯的研究和教育计划。本研究的目的是利用顺磁共振波谱技术研究二价金属离子在RNA和DNA聚合酶以及催化RNA分子进行的磷酸化转移反应中的作用。顺磁离子Mn（II）将取代Mg(II)，以便EPR和q波段ENDOR探针可以使用。生物物理研究将跨越生物、物理和分析化学的界限，探索核酸代谢的基本问题，为研究生和本科生提供跨学科的培训。生物主题将整合到本科物理化学课程中，并为研究生和高年级本科生开设生物物理/生物材料课程。

This CAREER Award in the Inorganic, Bioinorganic and Organometallic Chemistry Program supports the research and education plans of Victoria J. DeRose at Texas A&M University. The goal of the proposed research is to use paramagnetic resonance spectroscopic techniques to investigate the roles of divalent metal ions in the phosphoryl transfer reactions performed by RNA and DNA polymerases and catalytic RNA molecules. The paramagnetic ion, Mn(II), will be substituted for Mg(II) so that EPR and Q-band ENDOR probes can be used. Biophysical studies crossing the boundaries between biological, physical and analytical chemistry will be used to probe basic questions of nucleic acid metabolism and provide interdisciplinary training for both graduate and undergraduate students. Biological topics will be integrated into undergraduate physical chemistry courses and a biophysical/biomaterials course will be developed for graduate and upper level undergraduate students.