CAREER: Metal-Binding Sites in Nucleic Acids and Polymerases

这个职业奖在无机，生物无机和有机 化学计划支持维多利亚的研究和教育计划 德克萨斯A M大学的J. DeRose。 拟议研究的目标是 使用顺磁共振光谱技术来研究 二价金属离子在磷酰基转移反应中的作用 由RNA和DNA聚合酶和催化RNA分子进行。 的 顺磁性离子Mn（II）将取代Mg（II），使得EPR和 可以使用Q波段ENDOR探头。 生物物理学研究跨越了生物学， 物理化学和分析化学将用于探索基本问题 核酸代谢，并提供跨学科的培训， 包括研究生和本科生。 生物学主题将是 融入本科物理化学课程， 生物物理/生物材料课程将为研究生和更高 本科层次的学生。

This CAREER Award in the Inorganic, Bioinorganic and Organometallic Chemistry Program supports the research and education plans of Victoria J. DeRose at Texas A&M University. The goal of the proposed research is to use paramagnetic resonance spectroscopic techniques to investigate the roles of divalent metal ions in the phosphoryl transfer reactions performed by RNA and DNA polymerases and catalytic RNA molecules. The paramagnetic ion, Mn(II), will be substituted for Mg(II) so that EPR and Q-band ENDOR probes can be used. Biophysical studies crossing the boundaries between biological, physical and analytical chemistry will be used to probe basic questions of nucleic acid metabolism and provide interdisciplinary training for both graduate and undergraduate students. Biological topics will be integrated into undergraduate physical chemistry courses and a biophysical/biomaterials course will be developed for graduate and upper level undergraduate students.