CAREER: Metal-Binding Sites in Nucleic Acids and Polymerases

这个无机化学、生物无机化学和有机金属化学项目的职业奖支持维多利亚·J·德罗斯在德克萨斯农工大学的研究和教育计划。这项研究的目的是利用顺磁共振波谱技术研究二价金属离子在RNA和DNA聚合酶以及催化RNA分子进行的磷酸转移反应中的作用。顺磁性离子Mn(II)将取代Mg(II)，从而可以使用EPR和Q波段Endor探针。跨越生物、物理和分析化学之间的生物物理学研究将用于探索核酸新陈代谢的基本问题，并为研究生和本科生提供跨学科培训。生物学主题将被纳入本科生的物理化学课程，并将为研究生和高水平的本科生开发一门生物物理/生物材料课程。

This CAREER Award in the Inorganic, Bioinorganic and Organometallic Chemistry Program supports the research and education plans of Victoria J. DeRose at Texas A&M University. The goal of the proposed research is to use paramagnetic resonance spectroscopic techniques to investigate the roles of divalent metal ions in the phosphoryl transfer reactions performed by RNA and DNA polymerases and catalytic RNA molecules. The paramagnetic ion, Mn(II), will be substituted for Mg(II) so that EPR and Q-band ENDOR probes can be used. Biophysical studies crossing the boundaries between biological, physical and analytical chemistry will be used to probe basic questions of nucleic acid metabolism and provide interdisciplinary training for both graduate and undergraduate students. Biological topics will be integrated into undergraduate physical chemistry courses and a biophysical/biomaterials course will be developed for graduate and upper level undergraduate students.