Detection and amplification of DNA at near room temperature

近室温下 DNA 的检测和扩增

• 批准号: 1159841
• 负责人: Raj Mutharasan
• 金额: $ 34万
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2018-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1159841&HistoricalAwards=false
• 关键词: Detection; amplification; DNA; near; room

The overall goal is to investigate both detection and improvement of DNA using a device known as cantilever sensor. We will investigate systematically the role of intense surface vibration on bound double stranded DNA behavior. We hypothesize that the intense vibration will open up the molecule. Currently we do such a reaction by heating. Our preliminary work suggests that vibration directionally applied will cause DNA?s two parts to come apart. We will investigate if a method of increasing the DNA on the sensor can be accomplished. If these two goals are accomplished we may be able to detect DNA that is present in extremely small amounts. The implication of such a capability is we can detect harmful bacteria quickly saving lives; detect human and animal diseases rapidly and inexpensively. Intellectual Merits: Three merits are: (1) A new and cheaper approach will be developed for the detection of DNA at a very low level. (2) We will also develop a fundamental understanding of how vibration intensity affects DNA reaction, (3) Training of PhD students in the interdisciplinary area at the intersection of the broad field of nucleic acid chemistry (kinetics and thermodynamics), electromechanical resonators, and measurement science. Broader Impacts: Students of various backgrounds ? high school, undergraduates in various disciplines ? will be exposed to DNA measurement techniques. An important broad impact will be a new methodology that has sensitivity similar or better than the current expensive methods used in disease diagnosis and presence of harmful bacteria.

总体目标是使用一种名为悬臂式传感器的设备来研究DNA的检测和改进。我们将系统地研究强烈的表面振动对结合双链DNA行为的影响。我们假设强烈的振动会打开分子。目前我们通过加热来进行这样的反应。我们的初步工作表明，振动的方向性将导致DNA？S两部分解体。我们将调查是否可以实现一种增加传感器上DNA的方法。如果这两个目标都实现了，我们就有可能检测出极少量的DNA。这种能力的含义是，我们可以快速检测有害细菌，拯救生命；快速且廉价地检测人类和动物疾病。智力上的优点：三个优点是：(1)将开发一种新的、更便宜的方法来在非常低的水平上检测DNA。(2)我们还将发展对振动强度如何影响DNA反应的基本理解，(3)在核酸化学(动力学和热力学)、机电谐振器和测量科学等广泛领域的交叉学科领域对博士生进行培训。更广泛的影响：不同背景的学生？高中，不同学科的本科生？将会接触到DNA测量技术。一个重要的广泛影响将是一种新的方法，其灵敏度与目前用于疾病诊断和有害细菌存在的昂贵方法类似或更好。