SENSORS: Ultra Sensitive Continuous Tapered Fiber Biosensors for Pathogens and Bioterrorism Agents

传感器：用于病原体和生物恐怖主义制剂的超灵敏连续锥形光纤生物传感器

• 批准号: 0329793
• 负责人: Raj Mutharasan
• 金额: --
• 依托单位: Drexel University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0329793&HistoricalAwards=false
• 关键词: SENSORS; Ultra; Sensitive; Continuous; Tapered

0329793MutharasanThe goal of this research is to develop Antibody Sensitized Tapered Fiber Sensors arrays and Oligonucleotide Signatured Fiber Sensors for rapid direct detection and quantification of bioterrorism agents and their confirming DNA signatures using optical means with high specificity and sensitivity, and very low false positives. The proposed sensors will use evanescent absorption, scatter and fluorescence in continuous biconical tapered fibers for detection and quantification. The investigators will examine one- and multi- photon evanescent absorption, scatter and fluorescence for detection. The proposed sensors will not only measure the presence of bioterrorism agent(s) at very high sensitivity, but also establish their viability. The use of multiple strategies: evanescent absorption, scatter and fluorescence in single and multi-photon mode with the aid of surface immobilized monoclonal antibodies and unique DNA sequences specific to the target organism offers a comprehensive approach for detection. The broader impacts of this research are several-fold. The ability to detect and identify the presence or absence of pathogens or bioterrorism agents is essential for fighting terrorism. In addition, pin pointing the nature of the pathogen, especially its viability and virulence is paramount. If these can be accomplished expeditiously at the resolution of a single bacterium, the benefits to the society could be enormous. In addition, the sensors developed under this research can also be used for metabolic imaging of tissue in vivo. By positioning the tapers external to the tissue, the penetration of the 2-photon fluorescence excitation will enable the metabolic activity information from depths of several centimeters to be determined optically. A sequence of these tapers over the breast (as an example) can identify malignant tumors several centimeters beneath the surface.

0329793 Mutharasan本研究的目标是开发抗体敏化锥形光纤传感器阵列和寡核苷酸签名光纤传感器，用于快速直接检测和定量生物恐怖主义制剂及其确认DNA签名，使用具有高特异性和灵敏度的光学手段，假阳性率非常低。 所提出的传感器将使用渐逝吸收，散射和荧光连续双锥形锥形光纤的检测和定量。 研究人员将研究单光子和多光子的倏逝吸收、散射和荧光探测。 所提出的传感器不仅将以非常高的灵敏度测量生物恐怖主义制剂的存在，而且还将确定它们的生存能力。 多种策略的使用：在表面固定的单克隆抗体和对靶生物特异性的独特DNA序列的帮助下，以单光子和多光子模式的倏逝吸收、散射和荧光提供了一种全面的检测方法。 这项研究的广泛影响是多重的。 检测和确定病原体或生物恐怖主义制剂是否存在的能力对于打击恐怖主义至关重要。 此外，确定病原体的性质，特别是其生存能力和毒力至关重要。如果这些可以在解决单个细菌的情况下迅速完成，那么对社会的好处可能是巨大的。 此外，根据这项研究开发的传感器也可用于体内组织的代谢成像。 通过将锥体定位在组织外部，双光子荧光激发的穿透将使得能够光学地确定来自几厘米深度的代谢活动信息。 例如，在乳房上的一系列锥形可以识别出表面以下几厘米处的恶性肿瘤。