Development of a DNA amplification-free DNA detection assay.

开发无 DNA 扩增 DNA 检测方法。

• 批准号: 2432154
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2432154
• 关键词: Development; DNA; amplification; free; detection

DNA amplification techniques are essential for the study, detection and characterisation of a wide range of biological systems from Cancer to viruses. Polymerase chain reaction (PCR) is the most commonly used techniques but isothermal amplification approaches have also found use in recent years. These approaches rely on primer sets and enzymes to amplify target DNA sequences, followed by readout, typically using a fluorescent DNA binding dye. While these techniques are powerful they also have drawbacks in a range of environments either due to the need for specialised equipment or over sensitivity and none specific amplification.Here we propose to develop a novel, platform technology that allows for the study and detection of DNA (or RNA) without the need for specialised equipment or DNA amplification. Here acetoxymethyl ether modified dyes will be combined with catalytic entities to yield fluorescent signals in the presence of target genetic material.

DNA扩增技术对于研究、检测和表征从癌症到病毒的广泛的生物系统是必不可少的。聚合酶链式反应(PCR)是最常用的技术，但等温扩增方法在最近几年也得到了应用。这些方法依赖于引物组和酶来扩增目标DNA序列，然后读出，通常使用荧光DNA结合染料。虽然这些技术很强大，但它们在一系列环境中也有缺点，要么是因为需要专门的设备，要么是因为过于敏感和没有特定的放大。在这里，我们建议开发一种新型的平台技术，允许研究和检测DNA(或RNA)，而不需要专门的设备或DNA放大。在这里，乙酰氧甲醚修饰的染料将与催化实体结合，在目标遗传物质存在的情况下产生荧光信号。