Map-based cloning and functional validation of a QTL for grain size in wheat

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QTL Q.Tgw.ipk-7D increases grain size in wheat and also has positive effects on total grain mass and harvest index. By genetic methods, it was shown that a Mendelian gene inherited in recessive fashion is causing these effects. Our aim is the molecular identification and functional verification of the wheat gene underlying the QTL Q.Tgw.ipk-7D affecting grain size. The QTL interval was genetically delimited by fine mapping and synteny studies with rice and Brachypodium distachyon revealed a good synteny for the investigated region. The area of interest harbours 36 and 42 genes in rice and Brachypodium, respectively. Among them is a possible candidate gene for QTL Q.Tgw.ipk-7D encoding an AP2 domain containing protein. Further fine mapping is expected to narrow down the list of possible candidate genes for QTL Q.Tgw.ipk-7D. Therefore, the ongoing map based cloning approach is to be continued and obtained candidates are to be tested for their functionality in stably transformed wheat lines. The molecular identification of QTL Q.Tgw.ipk-7D will provide novel insight in the heritable regulators of grain size in wheat and would constitute the first cloned QTL reported in wheat.