Verification of a novel type of resistance genes conferring resistance to barley leaf scald caused by Rhynchosporium secalis

一种新型抗性基因的验证，该基因可抵抗由黑斑病菌引起的大麦叶烫病

• 批准号: 193306281
• 负责人: Dr. Marion Röder
• 金额: --
• 依托单位: Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/193306281?language=en
• 关键词: Verification; novel; type; resistance; genes

The goal of the proposed research is the functional verification of the Rrs2 gene rendering resistance against the fungal pathogen Rhynchosporium secalis in barley. In previous research the fine mapping of Rrs2 provided a genomic region cosegregating with Rrs2, which contained several predicted pectinesterase inhibitor genes and a number of other genes. We propose to establish a virus induced gene silencing system (VIGS) to test the cosegregating candidate genes functionally for their ability to break down Rhynchosporium resistance by RNAi. Additionally, the candidate genes for Rrs2 will be stably over-expressed in the Rhynchosporium-susceptible barley variety ‘Golden Promise’ and the transgenic lines analysed for their resistance. This research will lead to the functional characterization and verification of the Rrs2 gene, which potentially constitutes a novel type of resistance gene. After verification of the Rrs2 gene the expression pattern upon Rhynchosporium infection will be studied in detail.

该研究的目的是验证Rrs 2基因对大麦中真菌病原体Rhynchosporium secalis的抗性的功能。在以前的研究中，Rrs 2的精细定位提供了一个与Rrs 2共分离的基因组区域，其中包含几个预测的果胶酯酶抑制剂基因和一些其他基因。我们建立了一个病毒诱导的基因沉默系统（VIGS），以检测共分离的候选基因是否能够通过RNAi破坏Rhynchosporium抗性。此外，Rrs2的候选基因将在Rhynchosporium感病大麦品种'Golden Promise'中稳定过表达，并对转基因品系进行抗性分析。该研究将导致Rrs 2基因的功能表征和验证，这可能构成一种新型的抗性基因。在验证Rrs 2基因后，将详细研究Rhynchosporium感染后的表达模式。